运动与注射IGF-1对大鼠骨骼肌mTORAR蛋白表达影响.docVIP

运动与注射IGF-1对大鼠骨骼肌mTORAR蛋白表达影响 　　摘 要: 目的:观察运动、注射IGF-1对大鼠骨骼肌内IGF-1含量、mTOR、AR蛋白表达的影 响,探讨IGF-1对mTOR、AR的调节作用。方法:雄性成年SD大鼠24只,随机分为4组,安静对 照组(C)、安静+IGF-1注射组(CI)、运动组(E)、运动+IGF-1注射组(EI),每组6只。 运动方案为60 min/d,强度20 m/min,坡度10%,持续一周的跑台训练。每天训练后大鼠小 腿后侧皮下注射IGF-I,对照组注射安慰剂。最后一次运动后6 h取胫骨前肌,测定IGF-1 含量、mTOR、AR蛋白表达。结果:1) 与C组比较,E、EI组骨骼肌内IGF-1含量、mTOR蛋白 表达显著升高;2) C、CI、E、EI组骨骼肌内AR蛋白表达呈下降趋势。结论:1周耐力运动 可导致肌肉内IGF-1含量、mTOR蛋白表达显著增加,而肌肉中AR蛋白表达却呈现出下降趋势 ,其原因有待于进一步研究。 　　关键词:运动;骨骼肌;胰岛素样生长因子-1;哺乳动物雷帕霉素靶蛋白; 雄激素受体 　　中图分类号:G804.2文献标识码:A文章编 号:1007-3612(2010)05-0037-04 　　 　　The Effect of Insulin-like Growth Factor 1 (IGF-1) and Exercise 　　on the Protein Expression of ?Mammalian Target of Rapamycin (mTOR) and AndrogenReceptor (AR) in Rats Skeletal Muscle 　　HAO Xin?xin?1, ZENG Fan?xing?1, ZHAO Hua?2, OUYANG Qian?1 　　(1. Beijing Sport University, Beijing 100084, China;?2 . P.E. Department, Tianshui Normal University, Tianshui 741001, Gansu China) 　　Abstract: The study examined the effects of IGF-1 injection and exercise on the protein expression of mTOR, AR and IGF-1 level in rats skeletal muscle. 24 maleSprague-Dawley rats were randomly divided into four groups: inactive control(C),inactive plus IGF-1 injection (CI), exercise (E), exercise plus IGF-1 injection(EI). After all groups had been accustomed for one week, the Group E EI perf o rmed closely monitored training in the treadmill (grade of 5, at the speed of 20 m/min, duration of 1 hour) for 7 days. When the exercise programs were complete devery day, IGF-1 or placebo was injected subcutaneously in the back of right le g immediately. After 6 hours of the last exercise, Tibialis anterior muscle (TA )was isolated and IGF-1 level, the protein expression of mTOR and AR were determ ined. In our study, exercise evoked a significant (p0.05). The results indicate that one week endurance exercise stimu lu s induces an increase in IGF-1 level and mTOR protein expression. But the pheno menon of AR decrease should be taken further verification. 　　Key words: e