运动对胰岛素抵抗模型大鼠骨骼肌GLUT4和PKBβmRNA表达影响.docVIP

运动对胰岛素抵抗模型大鼠骨骼肌GLUT4和PKBβmRNA表达影响 　　摘 要: 目的:观察运动对胰岛素抵抗(Insulin Resistance,IR)模型大鼠胰岛素信号转导途径 中的关键蛋白GLUT4 (葡萄糖转运蛋4)和PKBβ(蛋白激酶Bβ)表达变化的影响。方法: 将健康Wistar雄性大鼠随机分为正常对照组、模型组、运动组及饮食干预组,每组8只。采 用高脂膳食喂养建立IR大鼠模型,分别以游泳运动和改饲普通饲料干预4周;测算胰岛素敏 感性指数(ISI);应用逆转录聚合酶链反应(RT-PCR)技术对各组大鼠骨骼肌细胞GLUT4和 PKBβ mRNA表达进行观测和定量分析。结果:运动显著提高IR模型大鼠ISI(?P? 　　关键词:胰岛素抵抗;运动;GLUT4; PKBβ;胰岛素信号转导途径 　　中图分类号:G804.7 　　文献标识码:A 　　文章编 号:1007-3612(2010)06-0044-03 　　 　　Mechanism study on Exercise’s effection on mRNA expression of G LUT4 and Akt2 in muscle cells of ?insulin resistance rats 　　BAI Zhen?min?1,WANG An?li?1,LI Sheng?zhi?2 　　(1.Sport Rehabilitation Department, Beijing Sport University, 　　Beijing 100084, China;?2.College of Preclinical Medicine, Heilongjiang 　　University of Chinese Medicine, Harbin 150040, Heilongjiang China) 　　Abstract: purpose: To develop a rat model of IR, and to study the effect of exer cise on expression of GLUT4 (glucose transporter 4) and PKBβ(protein kinase Bβ ) in somatic muscles of IR rats and the mechanism of acupuncture therapy in IR. Method: Male Wistar rats were randomly divided into 4 groups: The IR model rats without treatment group(M group), the IR rats with exercise group(E group), the IR rats with food intervention group(F group) and the normal control group(N gro up) and each group has 8 rats. Then the E group was treated with swimming exerci se and the F group was treated with common food for 4 weeks. 2. The blood sample s were collected to evaluate FINS, FBG and TG to calculate ISI (insulin sensitiv ity index). 3. The GLUT4 and PKBβ mRNA of skeletal muscle was determined with R T-PCR (reverse transcription-polymerase chain reaction). Results: exercise incr eased the IR rats’ ISI significantly(?P? 　　运动方法:有氧运动干预方法按Ploug等报道的游泳训练方法进行适当改进,运动组大 鼠在高脂喂养4周出现胰岛素抵抗后,进行游泳训练,每周训练5 d,前2周每天训练40 min , 以后每天训练60 min,保持水温35℃左右,水深50 cm,???只大鼠的活动面积200 cm?2,进 行为期4周的游泳训练。 　　1.3 胰岛素敏感性指数检测 　　在运动组大鼠给予运动治疗4周后,各组大鼠均禁食12 h过夜,次日空腹眼眶静脉窦采血 检测甘油三酯(TG)、空腹血糖(FBG)和血浆胰岛素(FINS)。FBG、TG