运动预处理对力竭运动大鼠大脑皮质GFAP表达影响.docVIP

运动预处理对力竭运动大鼠大脑皮质GFAP表达影响 　　摘 要: 目的:建立SD大鼠运动预处理模型和一次性力竭运动模型,探讨运动预处理对一次 性力竭运动诱导的大鼠大脑皮质神经胶质酸性蛋白表达的影响。方法:将30只SD雄性大鼠随 机分为3组:对照组(C组),力竭运动组(EE组),运动预处理组(EP组),EP组采用持续 4周的中等游泳负荷训练后,EE组和EP组负自身体重的3%进行一次性力竭运动游泳,用免疫 组织化学法及免疫印迹法检测大鼠大脑GFAP的表达。结果:EE组GFAP表达强度较C组无显著 性差异(?P?0.05),EP组较EE组及C组显著增强(?P? 　　关键词:运动预处理;力竭运动;大脑皮质;神经胶质酸性蛋白 　　中图分类号:G804.7文献标识码:A文章编 号:1007-3612(2010)10-0052-03 　　 　　Effect of Exercise Preconditioning on Exhaustive Exercise Induce d the Expression of 　　Glial Fibrillary Acidic Protein in Rats Cerebral Cortex 　　CHEN Yu,REN Xiu-qi 　　(Physical Education Department,Xi’an University of Arts and Sc ience, Xi’an 710000, Shaanxi China) 　　Abstract: Objective: Established SD rat’s exercise preconditioning models and exhaustiveexercise models to explore the influence of exercise preconditioning on exhausti ve exercise induced the glial fibrillary acidic protein expression in the cerebr al cortex of rats Method: 30 male SD rats were divided into three groups rando mly, control group (group C), exhaustive exercise group (group EE) and exercisepreconditioning group (group EP) First, group EP carries out a moderate exerci se preconditioning for 4 weeks, then group EE and group EP carry out an exhausti ve swimming loading with 3% body weight Immunohistochemical and western blotti ng ways were taken to detect the glial fibrillary acidic protein expression in t he cerebral cortex of rats Results: There were not significant difference of G FAP expression, the EE group and the C group(?P?0.05) However, there w ere significant difference when EP group vs both C group and EE group(?P? 　　1.3 灌注取材及标本制备 EE组及EP组大鼠进行一次力竭游泳, 游至力竭(鼻孔或大鼠整个没入水面以下5～10 s,不能自主上浮)并记录力竭时间,即刻 捞出水面,各组取5只大鼠,以10%水合氯醛(0.4 mL/0.1 g)腹腔麻醉,以4%多聚甲醛进 行灌注固定,获取大鼠大脑组织后以10%福尔马林液后固定,然后以30%蔗糖溶液浸泡脱水。 取出标??,快速冰冻切片,片厚10 μm,于-20℃保存。 　　1.4 免疫组织化学法检测GFAP表达小鼠抗大鼠GFAP单克隆抗体(Sigma公司)1:400稀释,严格按照SABC法试剂盒操作步骤进行染色,DAB显色,苏木素复染、中性树脂封片。各组内每只动物 连续取4张切片,在400倍光学显微镜下进行观察,并随机选取4个视野,采用Leica Qwin图 像分析系统,对各视野吸光度值(A)进行分析。 　　1.5 Western b