微阵列第1次课PPT.ppt

《微阵列设计与分析》第一章 绪论;微阵列技术起源;肿瘤基因组解剖计划;Once a Molecular Black Box;细胞的奥秘;细胞组成;DNA;Genes Functional Regions Within DNA Molecules;Genes “Switch On”;Gene to mRNA to Protein;Cancer – Genes With Design Problems;Expression Profiles;Expression Profiles in Cancer;Measuring Expression Profiles Step 1. Isolating the mRNA;Measuring Expression Profiles Step 2. Converting mRNA into cDNA;Measuring Expression Profiles Step 3. Converting the cDNA into a cDNA Library;Measuring Expression Profiles Step 4. Isolating Each Bacterium;Measuring Expression Profiles Step 5. Identifying the Clones;Active Gene to EST;C