自体冷血心脏停搏的液对未成熟心肌细胞atp及三磷酸腺苷酶的影响.doc

自体冷血心脏停搏液对未成熟心肌细胞ATP及三磷酸腺苷酶的影响 论着2010年7月第23卷第7期医学信息 iii※;!※jjjjiij;;;;;;;;ijjjjiii;;;;;;iii!※iiii;;;j;jjjiij;;;;::※;ii; 自体冷血心脏停搏液对未成熟心肌细胞ATP及三磷酸腺苷酶的影响 沈定荣王涛张青马超孟保英 【摘要】目的:通过对比自体冷血心脏停搏液与HTK液对未成熟心肌细胞ATP含量及三磷酸腺苷酶(ATPase)活性的影响,提示自体冷血心脏停 搏液对未成熟肌细胞的保护作用.方法:年龄≤1岁行体外循环室间隔缺损修补术的患儿4O例随机分为2组,实验组20捌,对照组2O倒.主动脉 阻断后,分别用自体冷血停搏液,HTK液根部灌注.于心脏停跳前,复跳后30min取右心耳标本50mg,测定心肌ATP含量及ATPase活性.运用统 计学t检验的方法,得出研究结果.结果:主动脉阻断前两组患儿心肌ATP含量(/tg/mg)与Na十一K+一ATPase,Ca+一ATPase,Ca2+一Mgz+～AT— Pase活一(~mol/mgoh)无统计学差异(Pgt;o.05);主动脉开放后30rain肌ATP含量与ATPase活性均较阻断前显着减少(Plt;0.01);主动脉开放 后30min两组惠儿心肌ATP含量与ATPase活性有明显差异(Plt;O.01);对照组主动脉开放后30rain心肌ATP的损耗量明显较实验组增多(Plt;O. 01).结论:自体冷血停搏液可提升体外循环内直视手术术后未成熟心肌细胞ATP含量及ATPase活性,对未成熟心肌的心肌保护效果明显优于 HTK液. 【关键词】自体冷血停搏液;HTK液;体外循环;能量代谢;未成熟心肌细胞 TheEffectofColdAutologousBloodCardioplegicSolutiontoATPContentsandATPaseActivitiesofImmatureCardiocyte SHENDing—rongW_ANG丁_noZHANGQingetal [Abstractl0bjective:ContrastingATPcontentsandATPaseactivitiesofimmaturecadiocytesaffectedbyautologousbloodcardioplegiesolutionand HTKsolution,istodemonstrateitsprotectiveeffectontheimmaturecadiocytes.Methods:4Ochildrenofageunder1一year—oldwithrepairofven— tricularseptaldefectincardiopulmonarybypasswereincludedandrandomlygroupedintotwounits:coldautologousbloodcardioplegia(n=2O;Ex— perimentGroup)andHTKsoJution(n=20;ControlGroup).ATPcontentsandATPaseactivitiesofRightatrialappendageweremeasuredpriorco asystole(Time1)and30minutesafterre—beating(Time2),witheachof(50rag)ofthespecimensbeingextractedrespectively.Bystudyingthe differencesinthetwogroupsofindicators,resultsareobtainedbytheuseofstatisticalttestmethod.Resuhs:AtTime1,ATPcontents(g/mg)and Na十一K+一ATPase,Ca+一ATPase,Ca+一Mg抖一ATPaseactivities(/~mol/mgoh)ofcadiocytesintwogroupshavenotstatisticaldifference(Pgt; 0.05).WhileatTime2,thedifferencebecomesignificant(Plt;O.01).ThecontentsandactivitiesdecreasesignificantlyatTime2inbothgroups(P lt;O.01).ThereductionOfATPcontentsisincreasedaftertheaortaunclampingincontrolgroup(Plt;0.01).Conclusion:ATPcontentsandATPase activi