酸枣仁汤对失眠大的鼠睡眠时相的影响.doc

酸枣仁汤对失眠大鼠睡眠时相的影响 【摘要】nbsp; 　　目的探讨酸枣仁汤对失眠大鼠睡眠时相的影响。方法在恒温、恒湿、自动光控及电磁屏蔽条件下，给予实验动物不同剂量酸枣仁汤，采用慢性电极埋植技术描记电刺激所致失眠大鼠自由活动情况下的皮层脑电图。分析给予实验动物不同剂量的酸枣仁汤前后失眠大鼠脑电图的变化。结果低剂量酸枣仁汤对失眠大鼠慢波睡眠Ⅰ期（SWS1）睡眠时相有显著延长作用（Plt;0.01），对慢波睡眠Ⅱ期（SWS2）没有明显影响（Pgt;0.05），中剂量酸枣仁汤能明显延长失眠大鼠SWS1 和SWS2（Plt;0.01），高剂量酸枣仁汤对失眠大鼠SWS2有显著延长作用(Plt;0.05)。结论酸枣仁汤对电刺激所致失眠大鼠的睡眠周期有影响，且随着剂量的变化作用不同。 【关键词】nbsp; 失眠大鼠 脑电图 睡眠时相 酸枣仁汤 　　AbstractObjectiveTo study the effect of suanzaoren decoction on sleep phase of insomnia rats. MethodsUnder the conditions of constant temperature, constant humidity, auto-light and electromagnetic shielding, we gave experimental rats suanzaoren decoction at different doses and adopted chronicity electrode embedment technology to trace the EEG of hypodermal layer of freerunning rats , which rececived electric stimulation. Then we analyzed the change of EEG of insomnia rats which were given suanzaoren decoction at different doses. ResultsThe results showednbsp; that suanzaoren decoction at low had significant lasting effect on SWS1 (Plt;0.01) and had no obvious effect on SWS2 (Pgt;0.05),the middle dose of it was able to extend SWS1 and SWS2 obviously (Plt;0.01) ,and the high dose of it could increase the duration of SWS2 obviously(Plt;0.05). ConclusionThe suanzaoren decoction can influence the sleep cycle of insomnia rats andnbsp; the effect are different with the the change of doses. 　　Key wordsInsomnia rats;nbsp; Electroencephalogram;nbsp; Sleep phase;nbsp;nbsp; Suanzaoren decoction nbsp;nbsp; 　　酸枣仁汤源自《金匮要略》“虚劳虚烦不得眠，酸枣仁汤主之”。长期临床观察结果表明酸枣仁汤或其加减方对各种失眠均有明显的改善作用。近几年来，开展了酸枣仁汤的现代药理实验研究，实验结果表明酸枣仁汤具有显著的镇静催眠作用［1～5］。为了进一步验证酸枣仁汤的改善睡眠作用，为临床应用酸枣仁汤治疗失眠提供实验依据，我们观察了酸枣仁汤对电刺激所致失眠大鼠的睡眠时相的影响。 　　1nbsp; 器材 　　1.1nbsp; 动物 nbsp; 　　Wistar大鼠，雄性，体重215～225 g（黑龙江中医药大学实验动物中心提供，许可证号：黑动字第。 　　1.2nbsp; 试剂 　　戊巴比妥钠（上海化学试剂采购供应站分装厂，批号020402，临用时以蒸馏水配制成1%溶液）；自凝牙托水（哈尔滨齿科器材厂，批号04-08-05）；注射用青霉素钠（哈药集团制药总厂， 批号。 　　1.3nbsp; 供试药品酸枣仁Ziziphus jujuba Mill. var.spinosa (Bunge) Huex H.F.Chou)、茯苓Poria cocos(Schw.) Wolf、川芎Ligusticum c