内毒素血症Wistar大鼠血清TNF—α与心肌细胞凋亡关系.docVIP

内毒素血症Wistar大鼠血清TNF—α与心肌细胞凋亡关系 　　[摘要] 目的 研究内毒素血症Wistar大鼠血清TNF-α与心肌细胞凋亡的关系及EPO对其的影响。 方法 将180只Wistar大鼠随机分为对照组、脂多糖LPS组及EPO处理组，分别在处理后2h、 4h、 6h、 12h、 24h断头取血并取其心脏。采用ELISA法测定血清TNF-α，用TUNEL法检测原位凋亡。 结果 ①LPS组TNF-α浓度在2h时达到最大值，且明显高于对照组（P0.05）。LPS+EPO组TNF-α浓度在2 h时达到最大值，且明显低于LPS组（P 　　[关键词] 内毒素血症；TNF-α；心肌细胞凋亡指数；EPO 　　[中图分类号] R541 [文献标识码] A [文章编号] 1673-9701（2014）02-0001-03 　　A study on the relationship between endotoxemia serum TNF-α of Wistar rats and apoptosis of myocardial cell 　　SUN Wei WANG Xiaoping JIN Wenyang 　　Emergency Department， Taizhou Hospital， Enze Medical Center of Taizhou in Zhejiang province，Taizhou 317000，China 　　[Abstract] Objective To study the relationship of rat serum TNF-α of endotoxin blood disease and the apoptosis of myocardial cells and EPO’s effects on it. Methods 180 Wistar rats were randomly divided into the control group， lipopolysaccharide LPS group and EPO treatment group. All the rats were respectively beheaded 2h， 4h， 6h， 12h and 24h after treatment for their blood samples and their levers were also taken out. Their TNF-α were tested by way of ELISA， and their in situ apoptosis were tested through the method of TUNEL. Results ①The TNF-α concentration of the LPS group reached its highest level in 2 hours， and it was obviously higher than that of the control group（P0.05）. The TNF-α concentration of LPS+EPO group reached its peak in 2 hours， and it was obviously lower than that of the LPS group （P 　　[Key words] Dndotoxin blood disease； TNF-α； Apoptosis index of myocardial cells； EPO 　　近年来研究证实，机体在遭遇感染时首先产生的、起核心作用的炎症介质是肿瘤坏死因子（tumor necrosis factor，TNF），尤其是TNF-α。脂多糖（LPS）是引起感染性休克机体损伤的主要物质，可对机体形成广泛的影响及损伤，并引起心率减慢、心肌收缩力下降，严重影响心功能[1]。已有研究表明[2]，EPO对心脏有保护作用。目前TNF-α与内毒素血症心肌细胞凋亡之间关系未见系统报道，外源性EPO对心肌的保护机制尚不清楚。本???究于2011年9月到2012年12月通过动物实验，采用ELISA法测定血清TNF-α，用TUNEL法检测原位凋亡，探讨血清TNF-α与心肌细胞凋亡的关系及EPO对其的影响，为促红细胞生成素在脓毒症患者中的应用提供理论依据。 　　1 材料与方法 　　1.1 实验动物及试剂 　　180只健康Wistar大鼠，不分雌雄，体重为（20±2）g，购买于华中科技大学同济医学院实验动物中心，许可证号为：SCXK（鄂）2004-0007。 　　EPO（产品序号CSB-E04539m）以及ELI