Genetic Diseases and Gene Therapy - Professor Mark …_26849：遗传性疾病和基因治疗_26849教授马克…….pptVIP

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2018-06-23 发布于浙江
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Genetic Diseases and Gene Therapy - Professor Mark …_26849：遗传性疾病和基因治疗_26849教授马克…….ppt

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Genetic Diseases and Gene Therapy - Professor Mark …_26849：遗传性疾病和基因治疗_26849教授马克……

Yesterday:Genetic Disorders and Gene Therapy Today:Genetic Engineering What does Genetic Engineering mean to You? Overview of Genetic Engineering What are the differences between cloning, recombinant DNA, and genetic engineering? What are the tools we use for genetic engineering? Plasmids Restriction Enzymes DNA Ligase Cloning Involves Making Identical Copies “Cloning” can mean several things: To make many identical copies of a DNA molecule or a particular stretch of DNA (DNA cloning or molecular cloning). To replicate an entire organism (reproductive cloning). Recombinant DNA Recombinant DNA is when two or more pieces of DNA that don’t normally go together are combined. Genetic Engineering Genetic Engineering is the process of using recombinant DNA technology to genetically alter or create new organisms. Recombinant DNA Technology Tools Plasmid Vectors To move DNA from one organism to another. Restriction Enzymes “Molecular Scissors” to cut DNA in specific places. DNA Ligase DNA “glue” to stick pieces of DNA together. Plasmids Small, circular pieces of “extra” DNA found in bacteria. Plasmids often carry antibiotic resistance. Plasmids Restriction Enzymes:Molecular Scissors A restriction enzyme (RE) is a specialized protein that cuts DNA in a very specific place. Different REs cut at different places along the nucleotide sequence. Cell DNA DNA Ligase (Glue) Recombinant DNA DNA fragment ligated (inserted) into vector creating recombinant DNA molecule. Today Gene Therapy Vector Engineering Project. Use paper models to simulate the process of engineering your recombinant gene therapy vector. DNA sheet for the therapeutic gene to treat the genetic disease you were assigned. DNA sheet for the vector you chose to treat the disease. Materials In lab folder (one per group): Activity Instruction Sheet (WHITE) Answer/Data Sheet (WHITE) Restriction Enzyme Sheet (GREEN) Get from me (one per group): Vector DNA sheet (PINK) Therapeutic DNA sheet (BLUE) Other: scissors/g