肠道感染病毒-微生物教学件_13.pptVIP

Disease Associations Paralytic Disease Meningitis Encephalitis Undifferentiated febrile illness Hand foot mouth disease Herpangina Epidemic Pleurodynia (Bornholm disease) Myocarditis Respiratory Infections Rubelliform rashes Neonatal Infection Conjunctivitis Pancreatitis/Diabetes Exanthems - Rubelliform rashes EV leading cause in summer fall. All types of rash Hand-foot-and-mouth disease Hand-foot-and-mouth disease: mostly coxackie A fever, malaise, sore throat, vesicles on bucal mucosa, tongue, hands, feet, buttocks highly infectious resolution – 1w Herpangina Herpangina – usually coxackie A acute onset, fever, sore throat, dysphagia lesions – posterior pharynx can persist w’s no gingivitis Laboratory Diagnosis Virus Isolation Serology Management and Prevention There is no specific antiviral therapy available against enteroviruses other than polio. IVIG HNIG There is no vaccine Echoviruses The first echoviruses were accidentally discovered in 1951 during epidemiological studies of polioviruses. CPE in cell cultures Department of Microbiology, Harbin Medical University * Medical Microbiology Department of Microbiology, HMU 第三篇 医学相关病毒 第31章 肠道感染病毒 Gastrointestine-Infected Viruses 微生物学教研室 钟照华 教学大纲 掌握内容 肠道病毒种类及共同特性 脊髓灰质炎病毒主要生物学性状、致病性、微生物学检查法及防治原则 柯萨奇病毒分组、分型及致病性 熟悉内容 埃可病毒、肠道病毒70型、肠道病毒71型的致病性 Morphology 27nm, icosahedral symmetry, no envelope Enterovirus structure Surface cleft – attachment to cellular receptors: Immunoglobulin superfamily, integrins, ICAM-1 肠道病毒特点 生物学性状 小球形病毒（~ 30 nm），无包膜 核酸为+ssRNA，有感染性 衣壳蛋白VP1-VP3分布在表面，VP4与RNA结合 在胞浆增殖，有明显CPE，破胞释放 耐酸耐乙醚，但鼻病毒除外 引起多种疾病 麻痹、无菌性脑膜炎、心肌损伤、腹泻、皮疹 脊髓灰质炎病毒（Poliovirus） First identified in 1909 by inoculation of specimens into monkeys First grown in cell culture in 1949 which became the basis for vaccines Transmission Fecal – oral route (粪－口途径) via hands and objects via food and water The first written record of virus infection A heiroglyph from Memphis, drawn in approximately 1400BC, which depicts a temple