小檗碱抗流感病毒性肺炎小鼠肺组织炎性损伤实验研究.docVIP

小檗碱抗流感病毒性肺炎小鼠肺组织炎性损伤实验研究 　　摘要：目的 观察小檗碱对流感病毒性肺炎小鼠肺组织中细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1 （VCAM-1）表达及炎性细胞渗出的影响，探讨其抗炎性损伤作用。方法 BALB/c小鼠108只随机分为正常组、模型组、小檗碱组。模型组和小檗碱组予25 ?L 50LD50病毒液滴鼻建立流感病毒感染的小鼠肺炎模型，感染后1 h，正常组和模型组予以双蒸水灌胃，小檗碱组每日予药物0.005 g/kg腹腔注射，各组均给药2次/d，连续给药5 d。感染后第2、4、6日分别取材，免疫组化法检测肺组织中ICAM-1、VCAM-1的表达，对肺泡灌洗液（BALF）中白细胞进行分类计数。结果 病毒感染后第2、4、6日，模型组肺组织中ICAM-1、VCAM-1的表达明显增强，小檗碱降低了ICAM-1、VCAM-1的表达水平，与模型组比较差异有统计学意义（P 　　关键词：小檗碱；病毒性肺炎；流感病毒；细胞间黏附分子-1；血管细胞黏附分子-1；炎性细胞；小鼠 　　DOI：10.3969/j.issn.1005-5304.2013.09.014 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2013）09-0040-04 　　Resistance of Berberine on Lung Inflammatory Injury in Mice with Viral Pneumonia Caused by Influenza Virus WU Ying1， LI Ji-qian1， MENG Jian2， LU Guang-lin1， HAO Yu1 （1.Department of Microbiology and Immunology， Beijing University of Chinese Medicine， Beijing， 100029 China；2.Beijing Royal Integrative Medicine Hospital， Beijing， 102209 China） 　　Abstract：Objective To observe the effects of berberine on ICAM-1， VCAM-1 expression and inflammatory cells exudation in mice with viral pneumonia caused by influenza virus， and explore its anti-injury effect. Methods Totally 108 BALB/c mice were randomly divided into control group， model group， and berberine group. 25 ?L 50 LD50 influenza virus， mouse lung-adapted strain， was intranasally inoculated to model group and berberine group. 1 h after infection， control and model group were intragastrically given 25 ?L distilled water， berberine group was treated by intraperitoneal injection with berberine at a dose of 0.005 g/（kg?d） for 5 days， twice per day. On day 2， 4 and 6 after infection， immunocytochemical method was used to detect ICAM-1 and VCAM-1 expression， and sorting cell count of leukocytes in bronchoalveolar lavage fluid （BALF） were counted. Results The expression of ICAM-1 and VCAM-1 in model group increased obviously on day 2， 4， 6， and which in berberine group decreased compared with model group （P 　　Key words：Berberine；viral pneumonia；influenza virus；ICAM-1；VCAM-1；inf