不同牙髓状态诱导型一氧化氮合酶表达的免疫组织化学分析-immunohistochemical analysis of inducible nitric oxide synthase expression in different pulp states.docxVIP
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不同牙髓状态诱导型一氧化氮合酶表达的免疫组织化学分析-immunohistochemical analysis of inducible nitric oxide synthase expression in different pulp states
山东大学硕士学位论文,,一,患者临床出现冷热刺激痛消除刺激后疼痛立即消失无夜间痛自发痛的患有,、,龋齿的第三磨牙例为牙髓充血组选取无系统性疾病牙周健康岁患者,。曾有夜间疼痛病史就诊时患者自觉疼痛不明显的检查发现龋洞阴影己与髓,,腔相连的第三磨牙例为炎症牙髓组,,,将以上三组牙齿收集完毕后立即用高速手机在冷水喷雾的情况下在每个、牙齿的近远中方向用裂钻钻出两条深沟然后用凿沿深沟将牙齿劈成两瓣用,。刮匙小心地将牙髓从牙齿中取出在粗糙的吸水纸上略定型后立即放入℃的,的多聚甲醛液中保存过夜将所有标本制作石蜡块实验,。将个的石蜡块沿纵轴方向制作成连续的厚约“的切片每一个标本选,,。张切片进行染色在镜下进行组织学观察选取经组织学观察分类后的牙髓,组织其余切片进行免疫组化染色光镜观察并拍片、结果、人正常健康牙髓组织中成牙本质细胞血管内皮细胞成纤维细胞都不表。、,达诱导型一氧化氮合酶,人充血牙髓中成牙本质细胞血管内皮细胞不表达诱导型一氧化氮合酶。,成纤维细胞存在诱导型一氧化氮合酶阳性表达,人炎症牙髓中一氧化氮合酶的表达存在于多型核白细胞成纤维细胞不,存在诱导型一氧化氮合酶阳性表达血管内皮细胞不存在一氧化氮合酶阳性表。达结论,诱导型一氧化氮合酶在正常组织中不表达说明诱导型一氧化氮合酶可作。为组织健康与否的标志物,,诱导型一氧化氮合酶在充血牙髓中的成纤维细胞表达由于成纤维细胞具,有分化为其他细胞的能力是
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