白藜芦醇对人神经母细胞瘤shsy5y细胞氧化损伤的保护作用及其机制研究-protective effect of resveratrol on oxidative damage of human neuroblastoma shsy 5 y cells and its mechanism.docxVIP

ABSTRACTIObjectivelToobservetheprotectioneffectsofresveratrolonSH·SY5YcellsoxidationdamageinducedbyH202．andtoexploreitspossiblemolecularmechanisms．fMethodslPDcellmodelwasestablishedbytreatingSH．SY5Ycellswith100pmol·L．1of}1202S}I-SY5Ycellswerepre-treatedwimdifferentconcentration(0．51amol。L．‘，5Brnol·L_‘，50prnol·L-1，1001xmol·L～，1251maol·L-1。200pmol·L-1)ofresveratrolfor2h,thenoxidationdamageWasindueedbyincubatingthecellswitll100l_tmol-L．1of}1202for8h。Afterthetreatment，cellviability,MDAconcentrationandGSH-Pxactivityofeachgroupwereexamined．mRNAexpression1evelofdifferentgenes(Bcl-2，Bax，CAT,nNOSandCOX-2)andproteinlevelofC0x．2werealSOmeasuredtounderstandunderlyingmechanism．IResultslTheeellviabilityofSH．SY5Ycellsdecreasedto18．35％afterbeingtreatedwith1001unol·L．1ofH202for8h。andtheeellnormalmorphouswerealsodisappeared．whichsuggesting也atPDcellmodelwassuccessfullyestablished．Pre—treatmentofresveratrolcouldeffectivelyincreasetheeellviabilityofSH．SY5Ycells．IntheresveratrolconcentrationrangefromO．51tmol·L—to1001amol·L～．theeellviabilityincreased丘om14．36％tothepeakof74．36％．From1001xmol·L“to200pmol·L．1．theeellviabilitygraduallydecreased．IntlleresveratrolconcentrationrangefromO．51amol·L-1to1001tmol·L一．themRNAexpressionlevelofBcl一2increasedwithincreaseofresveratrolconcentration．whilethemRNAexpressionlevelsofBax、CAT、IlNOS、COX．2andproteinlevelofCOX-2decreasedalongwiththeincreaseofresveratrolconcentration．1ConclusionlThePDcellmodelcouldbeestablishedbyinducingSH-SY5YoxidationdamagewithH202．ResveratrolcouldsignificantlyprotectSH-SY5YcellsfromoxidationdamageinducedbyH202．Rsveratrolmayexertprotectioneffectsviamodulatingtheexpressionlevelofseveralgenesrelatedtooxidativestressandapoptosis．RsveratrolmayhavetherapeuticpotentialinthetreatmentofneurodegenerationⅡdiseasessuchasParkinson’SdiseaseIKeywords】Resveratrol，neuroprotection,antioxidation，Parkinson’Sdisease，SH-SY5Ycell，oxidativedamagIlI原创性声明本人声明，所呈交的学位论文是本人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了论文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得中南大学或其他单位的学位或证