吡咯喹啉醌pqq对几种水生生物影响的分析-analysis of the influence of pyrroloquinoline quinone pqq on several aquatic organisms.docxVIP

Ab Abstract PAGE PAGE VI Abstract Pyrroloquinoline quinine (PQQ) is the third coenzyme following nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+), and flavin adenine dinucleotide (FAD) or flavin mononucleotide (FMN) in biological oxidoreduction. PQQ is not only a plant growth promoting factor but also an important nutrient for the growth, development and reproduction in the mammals. So far, the physiological functions of PQQ have been studied extensively developing both at home and abroad, but it is not clear whether it can play a physiological role to aquatic organisms. In this paper, four species of planktonic microalgae (Chlorella pyrenoidesa，Isochrysis zhanjiangensis, Nitzschia closterium and Scenedesmus quadricanda), Daphnia magna and Apostichopus japonicus were chosed as subjects to study the physiological effects due to the addition of exogenous PQQ, including that the growth of planktonic algae and its influence on the antioxidant capacity under H2O2 stress, the growth, development and reproduction of D. magna and its anti-starvation effect, as well as the coelomic fluid immune activity of A. japonicus. The results showed that: PQQ had no significant effect on the growth of four planktonic algae (p 0.05). For the microalgae cultured in the seawater (C. pyrenoidesa, I. zhanjiangensis, N. closterium), their antioxidant ability was not obviously changed by PQQ under H2O2 stress (p 0.05). But 1000 μmol/L PQQ can alleviate oxidative damage of S. quadricanda within 24 and 48 h, showing the higher cell density than that of H2O2 group (p＜0.05). 5 μmol/L of PQQ can significantly promote the reproduction of D. magna that total number of offspring increased from 159.5 to 207.67 (p＜0.05). With the increasing of concentration to 15 and 25 μmol/L, its effect of promotion was reduced. Compared to control group, PQQ failed to affect instars number and survival time (lifespan) of D. magna (p＞0.05). However, comp