背根神经节tresk参与神经病理性疼痛的作用分析-analysis of the role of dorsal root ganglion tresk in neuropathic pain.docxVIP

代背根神经节细胞TRESKmRNA及其蛋白的表达；TRESK基因上调可抑制辣椒素诱发的大鼠背根神经节细胞P物质的释放。4．鞘内注射TRESK基因重组腺病毒载体pAd/CMV/V5-DEST-TRESK对大鼠神经 病理性疼痛有治疗作用。其通过上调DRC中的TRESKmRNA和蛋白表达可抑制脊髓的 星形胶质细胞激活，达到治疗神经病理性疼痛的作用。关键词：神经痛；神经节；钾通道；TRESK；基因Study of effects on TRESK in dorsal root ganglion for neuropathic painAbstractBackgroundDRG(dorsal root ganglion) plays an important role in the pathogenesis of neuropathic pain. Many studies have shown that the variety of potassium channels subtype is essential for the beginning and development of neuropathic pain. TRESK(TWIK-related spinal cord potassium channels), which has been found recently, is expressed greatly in DRG. Its function is to regulate the resting potential of neurons and it is important in the process of excitability in neurons. More and more studies have proved that TRESK is likely to take part in the process of all kinds of pains. However, there aren’t any studies on whether TRESK of DRG plays a role in neuropathic pain.ObjectiveThe objective of this study is to observe that how TRESK mRNA change in the DRG firstly. After setting up the recombinant adenoviral vector containing TRESK gene, we transfect the DRG neurons, to verify the efficiency of transfection and regulation, and then to observe that how TRESK genes regulation affect the release of P substance in the DRG. In addition, after intraperitoneal injecte the recombinant adenoviral vector containing TRESK gene to rats, we aim to verify the effects on regulating TRESKmRNA and protein in DRG of the recombinant adenoviral vector containing TRESK gene, and to observe that how it affect the pain threshold of rats and the activation of spinal glial cells.MethodsThe experiment include four parts. Part Ⅰ:Thirty two male SD rats were randomly divided into 2 groups. They were respectively carried out shame operation(Group Shame, n=16) and spared nerve injure model (Group SNI, n=16). Eight rats in each group were sacrificed 1 day before operation, L4,5 DRG at operation side were isolated to determinate the