白香丹胶囊对pms肝气逆证模型大鼠的药理机制研究—含药血清对大鼠海马神经元5-htr2c的表达及其下游ip3dagca2信号通路的影响-pharmacological mechanism of baixiangdan capsule on pms liver-qi inverse syndrome model rats - effect of medicated serum on the expression of 5 - htr2c in.docxVIP

hippocampalneuronsandwedeterminedthechangesof[Ca2+]iwithFluo-3/AMcalciumfluorescenceprobe.Results:1.Thebehavioraltestresults:Comparedwiththenormalgroup,thescoresofthePMSliver-qiinvasionmodelgroupratsdisplaysignificantdifference(P0.001).Meanwhile,whentreatingwithdrugs,thesignificantdifferencedisappears(P0.01;P0.05;P0.01).Westernblotfindings:Comparedwiththenormalgroup,theproteinexpressionof5-HTR2CinPMSliver-qiinvationgroupincreasedobviously(P0.001).Comparedwithmodelgroup,allthreedruggroupsdecreasedsignificantly(P0.001;P0.05;P0.001).WealsofindtheconcentrationsofIP3/DAG/Ca2+inPMSliver-qiinvationgroupweresignificantlyhigherinneuronsthanthenormalgroup(P0.001;P0.001;P0.01).Aftertreatedwithdrugs,thecontentsofIP3/DAG/Ca2+inneonaterathippocampalneuronsweresignificantlyimproved(P0.05;P0.001;P0.05)andallofthemhadnosignificantdifferencecomparedwiththenormalgroup.2.ByRP-HPLCchromatography,wefindthatwhenthenormalratshadbeengivenBaixiangdanandPaeonialactifloraP.E.bygavagefor2hours,thepaeoniflorinlevelofthemedicatedserumwasmaximum.Afteractivatingthesignalingpathwaysof5-HTR2Cinhippocampalneurons,comparedwiththenormalgroup,theconcentrationsofIP3andDAGinbothBaixiangdangroupandBaishaogroupweresignificantlyreduced(P0.001;P0.001),butBaishaogroupwasnotasgoodasBaixiangdangroup.Also,[Ca2+]ibothinBaixiangdangroupandBaishaogroupwerelowerthanthenormalgroup(P0.001;P0.05).Conclusion:ThePremenstrualsyndrome(PMS)modelratswithliver-qiinvasioncanbepreparedsuccessfullybymultipleemotionalstimulations.5-HTR2CinhippocampalneuronsiscloselyrelatedtothepathogenesisesofPMSliver-qiinvasion.Baixiangdancapsulemedicatedserumcanchangetheexpressionof5-HTR2CandinfluenceontheconcentrationsofIP3/DAG/Ca2+incellularsignalingpathways.BaixiangdancapsulemightpartiallyplaysitsroleinamelioratingPMSbyregulatingtheexpressionof5-HTR2CandchangethecontentsofIP3/DAG/Ca2+.5-HTR2CmaybeoneofthedrugtargetsofBaixiangdancapsule.KeywordsBaixiangdancapsule;PMS;hippocampalneurons;medicatedserum;5-HTR2C目录引言-1-第一部分PMS肝气逆证模型大鼠含药血清对新生大鼠海马神经元5