磁性免疫层析技术在氯霉素残留快速检测中的应用分析-application and analysis of magnetic immunochromatographic technique in rapid detection of chloramphenicol residues.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文IIII燥对磁球展层的影响，发现干燥后的磁球更趋向于团聚。由于磁球 D 同小鼠 IgG 的偶联物，会发生团聚而影响展层，故最终选用磁球 A 开展氯霉素 残留检测方法研究。3) 建立了氯霉素残留的磁性免疫层析检测新方法：从磁球与抗氯霉素单抗的偶 联条件，T 点、C 点的包被条件，及免疫磁分离技术用于样品处理等条件， 对氯霉素的检测进行了优化。选择 CB 作为包被缓冲液，C 点的 GAM 最佳 包被浓度为 1:100 倍稀释，T 点为 1:5 倍稀释。磁球：mAbCAP 最佳偶联比为 100:1。100:1 的偶联比与 25:1 的比较，CAP 检测限由 10ng/mL 降低到了4ng/mL 以下。建立了使用磁分离的方法处理大体积样品的方法。本研究建立了一种将免疫磁分离与免疫磁层析相结合的磁性免疫层析快速检测 新方法，使用氯霉素作为检测对象，并与本课题组自制的光学成像分析仪相结合， 实现氯霉素残留的快速半定量检测。这些研究工作也将为今后发展免疫层析磁颗粒 的磁信号定量检测奠定基础。关键词：磁性纳米粒；免疫层析；氯霉素；快速检测；食品安全IIIIIIAbstractAlong with the frequent food safety incidents happening today, the food safety has become one emergent problem which more and more people concern about. Undoubtedly, this brings more challenges for the modern analytic techniques.Chloramphenicol, a broad-spectrum antibiotic, because of its good cost-effective ratio, was widely used for the bacterial disease in human beings and animals. Furthermore, the drug is also used as a feed additive in animal aquaculture. However, it has been proved that chloramphenicol would result in lots of side effects. Many countries prohibited the use of chloramphenicol in any edible species. The European Union reduces the detection limit from 10 μg/kg to 0.1μg/kg, 100 times stringent than the original standard; the US FDA also changed the detection limit from 5 μg/kg to 0.3 μg/kg. Thus, in order to control the prevalence of chloramphenicol, it is necessary to develop an efficient, sensitive and economic analysis method for chloramphenicol residues.The magnetic lateral flow immunoassays (MLFIAs), as a new analysis method, has replace the traditional marker colloidal gold to the magnetic bead (MB). MLFIAs can accomplish the quantitative detection by a material with special magnetic character.This paper intends to test several kinds of MBs, screen out the suitable MB for the MLFIAs and to optimize the conditions, establishing a new test system of MLFIAs. In this paper, we use both immunomagnetic separation and MLFIAs.The main works inclu