广西红斑蛤蚧化痰止咳平喘药效分析-analysis of the effect of guangxi erythema gecko on resolving phlegm, relieving cough and relieving asthma.docxVIP

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广西红斑蛤蚧化痰止咳平喘药效分析-analysis of the effect of guangxi erythema gecko on resolving phlegm, relieving cough and relieving asthma.docx

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广西红斑蛤蚧化痰止咳平喘药效分析-analysis of the effect of guangxi erythema gecko on resolving phlegm, relieving cough and relieving asthma

PAGE PAGE 10组比较有显著性差异(P＜0.01)。结论：1. 广西红斑蛤蚧的最大耐受量 MTD 为 32g 生药/kg 体重，相当于人临床日用量的 320 倍，毒副作用较小。2. 广西红斑蛤蚧具有止咳、化痰、平喘的作用。关键词：广西红斑蛤蚧；哮喘；急性毒性实验；药效实验The efficacy experiment of Cough relieving and Phlegm reducing and Asthma relieving of Guangxi erythema GeckoAbstractObjective: In order to provided the scientific basis for compared to the pharmacological function of natural Gecko, and experimental basis for further exploring the mechanism of asthma, this experiment was to observe the effect on acute toxicity, cough relieving, phlegm reducing and asthma relieving of the Guangxi erythema Gecko, through the experiment test, which was Basing on artificial breed.Methods: 1. The acute toxicity test: As the median lethal dose can not be measured by pre-experiment on mice by oral administration, the approach of maximum tolerated dose (MTD) was adopted in this experiment to determine the safety of its clinical application; and observed the response of mice in weight gain, and toxic deaths after administration. 2. Pharmacodynamic experiments: (1) Cough relieving experiment: cough models were induced by ammonia spray, and the frequency of mice’s cough and incubation period were recorded. (2) Phlegm reducing experiment: The method of airway excretion of phenol red was adopted. 0.5% phenol red solution was intraperitoneally injected, tracheal lavage fluid was collected, and OD values were measued with a spectrophotometer. (3) Asthma relieving experiment: Asthma models of mice were sensitized with Ovalbumin(OVA), and the mices peripheral blood EOS and changes of total IgE levels in serum were counted to observe the effects of asthma relieving of Gecko.Results: 1. The determination of Guangxi erythema gecko’s maximum tolerated dose: within 14 days, activities of the mice in each group werenormal, no abnormal phenomenon, and no deaths were recorded. Mice in the control group and the treatment group were in the normal growth of body weight. There is no significant difference in ave