马铃薯块茎贮藏期抗性淀粉与蔗糖转化酶活性的相关性.docVIP

PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT 21马铃薯块茎贮藏期抗性淀粉和蔗糖转化酶活性的相关性学校名称：内蒙古农业大学作者姓名：陆晓玉指导老师：霍秀文摘要本实验材料均为内蒙古田间统一收获的马铃薯块茎，选用中薯10号，中薯17号和费乌瑞它三个品种，收获后直接入窖贮藏，窖温保持在4摄氏度。三个品种都采用压热法测定抗性淀粉含量，用3,5-二硝基水杨酸比色法测定淀粉-糖代谢酶中蔗糖转化酶的活性动态变化，通过数据比较的方法，研究马铃薯抗性淀粉和蔗糖转化酶活性的相关性。结果发现：通过块茎中蔗糖转化酶活性与抗性淀粉含量的相关性可知，马铃薯块茎中的蔗糖转化酶活性与抗性淀粉含量有显著的相关性，其中费乌瑞它（R=－0.988）,中薯10号相关系数为（ R=－0.854），中薯17号相关系数为（R=－0.999）。三个马铃薯品种均呈负相关。即蔗糖转化酶活性越强，则抗性淀粉含量越低。关键词：马铃薯；贮藏期；抗性淀粉；蔗糖转化酶活性Abstract The?experimental?materials are?Inner Mongolia field?uniform?harvest?of potato tubers in selection?in?potato,?10?potato,?17 and?Favorita?three varieties,harvested directly into the storage pit,?pit?temperature?maintained at 4 degrees celsius.?Three species?are?used for the determination of?resistant starch content in?hot press?method,?determination of?the dynamic changes?of the?activity of?invertase?of starch sugar metabolic enzymes?by 3,5- two?nitro salicylic acid?colorimetry?method,?through the?data comparison,?correlation of?Potato?Resistant Starch?and sucrose?invertase?activity.?The results showed: Through the correlation?that?invertase?activity and?the resistant starch content of?tuber,?from potato tuber?invertase?activity and?resistant starch content?had?significant correlation,?which Faye U Ray Tia?(R= 0.988),?in the potato 10?correlation coefficient?(R= 0.854),?in the potato 17?correlation coefficient?(R=0.999).?Three potato varieties were negatively correlated.?That is,?the stronger the?invertase?activity?is?lower,?the resistant starch content.Keywords: potato; storage period;?resistant starch;?invertase?activit目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc24330 1引言 PAGEREF _Toc24330 6 HYPERLINK \l _Toc3677 1.1 马铃薯淀粉 PAGEREF _Toc3677 6 HYPERLINK \l _Toc19487 1.2马铃薯抗性淀粉 PAGEREF _Toc19487 6 HYPERLINK \l _Toc12118 1.3蔗糖转化酶 PAGEREF _Toc12118 8 HYPERLINK \l _Toc28975 1.4本实验研究的目的和意义 PAGEREF _Toc28975 8 HYPERLINK \l _Toc26203 2马铃薯抗性淀粉含量和蔗糖转化酶活性的相关性分析 PAGEREF _Toc26203 10 HYPERLINK \l _Toc7969 2.1材料与方法 PAGEREF _Toc7969 11 HYPER