放射性脑损伤模型文章汇总摘要.docxVIP

2016 Neuroprotective effects of human umbilical cord–derived.pdfThe irradiation procedure has been described previously[21]. Briefly,WBI was performed using a 60Coγ-rays irradiator using lead shielding devices so that the whole brain, including the brainstem, was irradiated,while the rest of the body was shielded. Irradiated mice received a single dose of 15 Gy at a dose rate of 1.17 Gy/min. Sham-irradiated control mice were anesthetized but not irradiated. Body weight was measured weekly during the experimental course (12 weeks).钴60 全脑包括脑干 一次剂量15Gy 12周后安乐死2015 Extracellular ATP enhances radiation-induced brain injury through.pdf2.5. Cell culture and irradiationPrimary microglia derived from newborn mice were prepared from mixed glial cultures using the ‘‘shaking off’’ method, as described previously (Suzumura et al., 1987). Briefly, pups were sterilized with 70% ethanol, the brain tissue was placed on ice cold HBSS. The meninges, cerebellum and brain stem were removed under a dissection microscope. The remaining tissue was chopped into small pieces, washed with serum-free DMEM and then digested with a mix of papain (30 U/ml), Dnase I (200 lg/ml),EDTA (0.5 mM) and L-cysteine (0.2 mg/ml) for 30 min at 37 C.The mixture was then spun down; the pellet was carefully suspended in DMEM with 10% fetal bovine serum and cells transferred into a T75 flask. After 24 h the medium (DMEM supplemented with 10% fetal bovine serum, 1 mM Na + pyruvate, 100 units/ml penicillin,and 100 lg/ml streptomycin) was changed. The cells were cultured until rounded, shiny microglial cells started to float off the astrocyte layer. These cells were shaken off, collected, washed with DMEM for further experiments. The primary cultured neurons and astrocytes were harvested from the hippocampi of postnatal day 0 embryonic mice, as reported previously (Wang et al.,2011). The cells were randomly divided into four groups: control, radiation, ATP, radiation with ATP pre-treatment, and radiation with BBG