2―甲氧基雌二醇联用抗坏血酸诱导慢性粒细胞白血病细胞凋亡及细周期变化研究.docVIP

2―甲氧基雌二醇联用抗坏血酸诱导慢性粒细胞白血病细胞凋亡及细胞周期变化研究 　　[摘要] 目的 研究2-ME联合抗坏血酸在诱导CML细胞凋亡中的作用以及细胞周期的变化机制。 方法 收集2016年1～12月期间汕头市中心医院血液科已确诊的bcr/abl（+）慢性粒细胞白血病患者57例骨髓，应用流式细胞术分选出CML细胞并培养传代并随机分为四组。使用抗肿瘤化合物2-甲氧基雌二醇（2-ME）与抗坏血酸（AA）组合来处理慢性粒细胞白血病（CML）细胞，根据药物浓度分为：（1）未加药组；（2）2-ME组（2?mol/L）；（3）AA组（62.5?mol/L）；（4）2-ME联用AA组（2-ME：2?mol/L+AA：62.5?mol/L）四组。应用流式细胞术分析药物处理后的CML细胞凋亡情况和细胞周期的变化情况。结果用（）表示，两组间比较采用t检验，多组间比较用F检验。结果 2μmol/L 2-ME +62.5μmol/ LAA组可诱导CML细胞凋亡。 2-ME+AA以剂量依赖性方式诱导凋亡，在浓度2～8μmol/ L时凋亡呈时间-剂量依赖。2-ME+AA可诱导处于G0/G1期的细胞明显增多，处于S期的细胞减少。 结论 2-ME联合抗坏血酸可诱导白血病细胞以时间-剂量依赖式凋亡，且与细胞周期抑制有关。 中国论文网 /1/viewhtm 　　[关键词] 白血病细胞；2-ME；抗坏血酸；流式细胞术；凋亡；周期变化 　　[中图分类号] R733.7 [文献标识码] A [文章编号] 2095-0616（2017）11-18-04 　　Study on apoptosis and cell cycle changes of CML cells induced by 2-Methoxy estradiol combined with ascorbic acid 　　ZHANG Suwei ZENG Chang LIN Xiangwei 　　Department of Laboratory， Shantou Central Hospital， Shantou 515031， China 　　[Abstract] Objective To study the role of 2-ME combined with ascorbic acid in the induction of CML cell apoptosis and the mechanism of cell cycle. Methods 57 Bone marrow of patients who diagnosed bcr/abl（+） chronic myeloid leukemia in Department of Hematology， Shantou Central Hospital between January 2016 and December 2016， was collected.And CML cells were sorted out by flow cytometry.CML cells were randomly divided into four groups.2-methoxy estradiol（2-ME） combined with ascorbic acid （AA） was used to treat chronic myeloid leukemia（CML） cells. According to the drug concentration is divided into four groups：（1）control；（2）2-MEgroup（2?mol/L）；（3）AA group （62.5?mol/L）；（4）2-ME combined with AA group（2-ME：2?mol/L+AA：62.5?mol/L）. Flow cytometry was used to analyze the apoptosis and cell cycle of CML cells. Results CML cell apoptosis was induced by 2μmol/L 2-ME+62.5μmol/L AA group. 2-ME+AA induced apoptosis in a dose-dependent manner， and apoptosis was time-dose-dependent at the concentration（2-8μmol/L）. 2-ME+AA can induce cells in G0/G1 phase increased significantly， in th