猕猴源肺炎链球菌分离和鉴定及fqpcr建立和在阐明其侵染机制研究中的应用-isolation and identification of streptococcus pneumoniae from macaca mulatta and establishment of fq pcr and its application in elucidating its infection mechanism.docxVIP

lmWIllmmmimmmY1801U4目录本文创新性研究工作.............…..............................................................…................1111材料与方法................................................………....…川…..…………........….........51.1试验材料，..……...............，..…..............….......................................…..............….........51.1.1菌株................................................川............川..........川..…………………川...51.1.2试瞌动物…….......川........................….......…...............….........…………..........51.1.3主要试验仪捕……………川……........................川.........川…..……..........…….51.1.4主要购买试制及其它配制试制.......……川.........……..........……..........….....51.2狲猴源肺炎链球菌商株的分离和接定....................................………·川..........…..川....71.2.1狲猴源肺炎链球菌菌株的分离....................……..........................................…71.2.2狲猴源肺炎链球菌商株的鉴定....……......…..川…川…..........川…..1.立2.2.1革笠氏染色镜检…….....川…....…....…..…………..…H…....…....川…....…………..………..……...川…...…..胆汁榕解试验川……..，.川….川川..…….……...…..，.川……川..，.，川.….….，.…....…...，…川.….....川…....……..，.，.，.……·………..………奥谐托欣敏感试验............………………...........，..............…............…..川..小鼠毒力试验.........，..……......，.........…........…川……........………....，...，……...71.3肺炎链球菌种特异性常规陀R方法的建立….....川川.….…......…....…..川…...川…...…，....川川….1.3.1常规PCR引引|物的设计…川….……..，川…...，…....….，..…....川…川..….....川.……......川川.……......川川.….….....川.….…...……..川….……，..…81.3.2细菌t培音葬.........….............….....................................…………川........叫…..…..81.3.3细菌基因组DNA的提取....…...........………….........川…·川…......……....…91.3.4常规PCR反应的罐立与优化…......................…·…..川......川…川…....……....川…91.3.5常规PCR的特异性试式验H川…川..…...川…...…川…...…..…1.4肺炎链球菌种特异性FQ.PCR方法的建立……....…....…..…….川…川.…....川….1.4.1定量PC侃R号引|物.川.…....…....….....…......…....…..川…...川.…...川…...........…...……·………91.4.2FQ.PCR标准晶目的DNA的的制备..............………...................………川...101.4.3定量PCR的建立及反应条件的优化……....…....川…....…....川川.……....1.4.4定E蟹PCR标准晶目的DNA棋板浓腹的测定…川….川….川.川..…..川….……川….川….…川….…lω01.4.5定量PCR栋准曲绒的制f你售….川.川.…..….…..川川.….…...