麻杏芎葶合剂对支气管哮喘大鼠肺组织pkc、mmp-9影响的实验分析------附典型临床病例-experimental analysis of the effect of maxing xiongting mixture on pkc and mmp - 9 in lung tissue of bronchial asthma rats with typical clinical cases.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 中文摘要 第一部分：动物实验：实验目的：观察中药麻杏芎葶合剂对支气管哮 喘大鼠肺组织蛋白激酶 C(PKC)、基质金属蛋白酶-9（MMP-9）表达水平的影响， 探讨麻杏芎葶合剂治疗哮喘大鼠气道炎症的作用机制。 方法：取 SD 大鼠 60 只，随机分为空白对照组、哮喘模型组、麻杏芎葶合剂低、 中、高剂量组、地塞米松组。除空白组外，其他 5 组均行哮喘造模，麻杏芎葶合 剂不同剂量组分别给予相应剂量的药液灌胃，模型组给予等量生理盐水，地塞米 松组给予相应剂量地塞米松腹腔注射。采用免疫组织化学法观察各组大鼠肺组织 PKC 及 MMP-9 表达的变化，并光镜观察肺组织病理变化。 结果：麻杏芎葶合剂可能通过抑制哮喘大鼠 PKC 及 MMP-9 的表达抑制炎性细 胞的分泌，从而达到调节 Th1/Th2 细胞因子平衡，减轻气道炎症，干预气道重塑， 缓解哮喘病情。 第二部分：麻杏芎葶合剂之理论探讨 第三部分：综述 关键词：支气管哮喘 麻杏芎葶合剂 大鼠 PKC 及 MMP-9 理论探讨 综述 Part I：Animal experiments： Abstract Objective：To observe the expression of Ma Xing Xiong Ting mixture of PKC and MMP-9 in bronchial asthma lung tissue and explore the Ma Xing Xiong Ting Continuous Mixture on airway inflammation in asthmatic rats and its mechanism study. Methods：SD 60 rats were randomly divided into control group, asthma group, the Ma Xing Xiong Ting mixture of low, medium and high dose group, dexamethasone group. In addition to the control group, the other five groups underwent asthma models, Ma Xing Xiong Ting mixture three groups were given different doses of the appropriate dose of liquid medicine orally, the model group were given normal saline and dexamethasone groups were given the same dose of dexamethasone intraperitoneal injection.Observing by immunohisto chemistry the expression of the PKC and MMP-9 in lung tissue of rats and the changes of lung tissue pathology by light microscopy. Results：Ma Xing Xiong Ting mixture may inhibit secretion of inflammatory cells by inhibitting the expression of PKC and MMP-9 in asthmatic rats，So as to achieve regulating Th1/Th2 cytokine balance to reduce airway inflammation and airway remodeling interventions and alleviate the asthma situation. Part II：the theoretic discussion on “Ma Xing Xiong Ting combination”。 Part III：Summary. Key words：Bronchial asthma Ma Xing Xiong Ting combination Rats PKC and MMP-9 Theoretic discussion Summary PAGE PAGE 1 英文缩略词表 ACRONYMS AND ABBREVIATIONS 缩略词 ACT 英文全文 Asthma Control Test 中文 哮喘控制测试 AHR Airway Hyperresp