浅刺针法对脑梗塞大鼠脑组织缺血半暗带fas、bcl-2蛋白表达影响-effect of shallow needling method on fas and bcl - 2 protein expression in ischemic penumbra of cerebral infarction rats.docxVIP
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浅刺针法对脑梗塞大鼠脑组织缺血半暗带fas、bcl-2蛋白表达影响-effect of shallow needling method on fas and bcl - 2 protein expression in ischemic penumbra of cerebral infarction rats
TheshallowthornneedlelawischemiahalfblankingbartothecerebralinfarctionratbrainorganizationFas,theBcl-2proteinexpressioninfluenceABSTRACTObjective:TostudyshallowSpikeActofratbraintissueaftercerebralinfarctionischemicpenumbraofFas,Bcl-2effect.Methods:60SDratswererandomlydividedintoshallowSpikeAct(Ⅰ),conventionalacupuncturegroup(Ⅱ),themodelgroup(Ⅲ),andnormalcontrolgroup(Ⅳ).Thromboembolismthreegroupsusingtheratmodeloftheestablishmentofcerebralinfarction.Ⅳgroupwithoutanytreatment,butatthesametimebundledfixed.Theneachgroupwererandomlydividedintothreedaysafter2,7,14pointintime,eachofthefivetimepointsinrats.Neuralsignsfromthescore,usingimmunohistochemicalmethodsofbraintissueFas,Bcl-2proteinexpressionchangesobservedafter2,7,14daysaftertreatmentⅠ,ⅡratsandthecorrespondingtimeuntreatedIII,IVratbraincellapoptosis.Results:ThreetimepointsshallowSpikeActratbrainischemicpenumbrafasproteinexpressionwassignificantlylowerthanthemodelgroupandtheconventionalgroupacupuncturegroup,andBcl-2wassignificantlyhigherthanthatmodelandtheconventionalgroupneedlethorngroup(P0.01),indicatingthatshallowSpikemethodcanbeeffectivelyimprovedcerebralinfarctioninratsnervedefectsigns.Conclusion:Fas,Bcl-2inratbrainischemiccerebralinfarctioncellapoptosisplaysanimportantrole,thornshallowditch,whichcustomsveryrenzhong,neiguan,jiquan,cizhe,SanyinjiaopointreductionthroughincreasedandexpressionofFasproteinBcl-2proteinexpression,inhibitionofapoptosis,aneuroprotectiveeffect.AndMeridianconfirmedatthemolecularlevelinthebodyhasitsmaterialfoundation,shallowthornscanbeaneffectivestimulustoshallowMeridianspecificorganizationscanfullydisplayed.Keyword:Shallowthornneedlelaw;Cerebralinfarction;Halfblankingbar;Fas;Bcl-2引言脑血管病是严重威胁人类健康的常见病,是目前危害人类健康的三大疾病之一,我国每年死于心脑血管疾病的患者占死亡总数的第一位。[1]除成人卒中急性期溶栓治疗外,其他治疗脑梗死的药物均未取得满意的临床疗效。因此对本病的治疗仍是国内外研究的热点。针灸是脑梗塞的有效疗法之一。而采用毫针浅刺针法则少有报道。范郁山导师在总结前人及多年研究的基础上,运用浅刺针法治疗脑梗塞疾病取得了较好的临床疗效。[2]认为经络在肌体浅层有其物质基础,浅刺亦能有效地刺激经络和穴位,应该重视经络学说中的皮部、络脉理论的应用研究,以提高针灸治疗效果。在这一理论指导下,本研究拟运用免疫组织化学方法,从浅刺针法对大脑中动脉栓塞(MCAO)模型大鼠脑组织缺血半暗
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