肉桂醛与柠檬醛抗hl-60细胞生长效果及机理比较分析-comparative analysis of cinnamaldehyde and citral anti-hl - 60 cell growth effect and mechanism.docxVIP

IV IV on the HL-60 cell growth; （4） Application of AO/EB staining, morphological observation by scanning electron microscopy, transmission electron microscopy method to observe the morpho -logical changes of cell morphology,to reveal the two aldehyde on the cell line HL-60 of a single standard rules of changes. （5） Flow cytometry testing cinnamaldehyde and citral on apoptosis rate of HL-60 cells to the determination of mitochondrial membrane potential, intracellular ROS production volume, the change of metabolism on the role of the two aldehyde on the cell line HL-60. Results: Cinnamaldehyde and citral were inhibited HL-60 cell growth and induced apoptosis in biological effects, while the former than the latter good selectivity, low concentrations of cinnamaldehyde can be found through the membrane into the cell damage that increased mitochondrial oxygen consumption rate; can induce apoptosis, inhibit the growth of HL-60 cells.By the determination ROS, Although the latter process of apoptosis is similar, the former is presented through the cavity of the cells scattered, leading to nuclear apoptosis is a contraction, followed by the same drug dose is the amount of apoptosis, but the former is less than the latter, excluding cells of inflammation，To achieve from a single cell and subcellular level in real time, in situ, without disturbing the state of trans-cinnamic aldehyde interpreting anti-HL-60 cells, the mechanism for its initial clinical application of theoretical basis and technical parameters. Key words: cinnamic aldehyde, citral, HL-60 cells, growth inhibition, apoptosis 独创 性 声明 军人唱阴里交 的论文是钱个人在尊 师宿导下边好纳 研究工作11取得的研究成果.尽夜月号 - -了支由得剿篇以标注拥戴峭的地方外.论文 中不包含其他人已经发程虫写过的哥究成果 . -不包含i震撼安锺农业大学或其它很商机构的 学缸.佳节而使用泣的材料.与我- 工作的问 !%P岖$í傲的任何费M均已在论文中悻了明确的说明并囊示了甜雹 . . :蒋 钱 r;(iiJ . Oi/ 每 6叫E 关于论文使用授权的说明 本人宪全了. 徽农业大学有热保留.使用学位怆文 的规定.固自学段有貌镇锢缉交论文的 ，. ?件事.盒1ft许诠文革董倒和倩阅 .可以采用摩甸、甸回a2归楠只御手民愣鄂 、现 锚学位 x. 罔霆安疆农业大李可以用不何方式在不回健体上发现 .佬鸣曲也论文到金串成锦分内事. {镖的学位论 文在解密局应遵守 此协议} 究生如 扣乏一 四川01/ 年