水溶性含糖荧光探针分子刷的制备及生物应用分析-preparation and biological application analysis of water-soluble fluorescent probe molecular brush containing sugar.docxVIP

AbstractWater-solubleconjugatedpolymerbrushhasgoodwatersolubility,opticalstabilityandbiocompatibility,andbeingin-depthstudiedinthefieldofbiologicalandchemicalsensing.Theanionicpolymerbrushhasacertaindifficultytoenterintothecellmembrane,andthecationicpolymerbrushhasahighbiologicaltoxicity.Forthisreason,wehavesynthesizedaneutralstructureofthelactosewater-solubleconjugatedpolymerinthisthesisandstudiedit’smolecularstructurethroughtheUVabsorption,fluorescenceemissionspectra,dynamiclightscattering,scanningelectronmicroscopyandtransmittanceofelectronmicroscopy.Wewanttoobtainanidealfluorescentmaterialwithpropertiessuchasgoodwatersolubility,highfluorescencequantumefficiencyandgooddispersion,etc.Meanwhile,wehopetostudytheapplicationofthismaterialinbiologicalsensingandmetalionsdetection.Thisthesisisdividedintofourpartsasfollows:1,Wesynthesizedawater-solubleconjugatedpolymerbrushcontaininggalactose(PFB-Gal).Firstlywesynthesizedapolyfluorenebenzenemacromercontainingside-chainhydroxylgroupsbySuzikiCouplingReaction,thenwithdibromo-isobutyrylbromide,esterificationreactionwascarriedoutundertheconditionoflowtemperaturetoobtainamacroinitiator,afterthat,themacroinitiatorreactedwithagalactosemonomerwhichisundertheprotectionofthehydroxylgroupviatheatomtransferradicalpolymerization(ATRP)toobtainabrush-typeconjugatedpolymercontainingpolylactose.Afterthehydrolysisreaction,weobtainedawater-solubleconjugatedpolymerbrushcontaininggalactose(PFB-Gal).ThestructureofthesynthesizedPFB-GalwascharacterizedwithFTIRandNMR,andtheresultsshowedthatwehavesuccessfullysynthesizedanovelwater-solubleconjugatedpolymers.2,Thewater-solubleconjugatedpolymercontaininggalactose(PFB-Gal)hasgoodbiocompatibility,goodwatersolubility(38mg/mL)andfluorescencestability.Theabsorptionandemissionpeakoffluorescencewerelocatedat350nmand445nm,respectively.Thefluorescenceefficiencyis54%.PFB-Galwasresistanttometalions,andit’sfluorescencewasverystableinanacidicenvironment.Thetestsoftransmissionelectronmicroscope(TEM),anddynamiclight