四种生血法对骨髓抑制小鼠血清tpo epo gm-csf及骨髓cd34影响的比较研究-comparative study on the effects of four blood-generating methods on serum tpo epo gm - csf and bone marrow cd34 in mice with bone marrow suppression.docxVIP

补肾生血法和调肝生血法能促进骨髓抑制小鼠造血功能的恢复，在恢复外周血象，调控血清TPO、EPO、GM-CSF水平，增加骨髓CD34+细胞数量，修复骨髓组织方面具有更明显的优势。关键词：调肝生血；补心生血；补脾生血；补肾生血；60Co-γ射线；骨髓抑制3ABSTRACTObjectives：Toobservethetherapeuticeffectofthehemopoieticmethodsofregulatingliver，tonifyingheart，tonifyingspleenandtonifyingkidneyonmarrow-suppressedmiceafterradiation，compareandstudytheregulatoryeffectofpromotinghematopoieticfunctionandthepossiblemechanismsofmarrow-suppressedmice.Toprovidetheoreticalandexperimentalstudyevidenceforproperandreasonableutilizationofthehemopoieticmethodsinclinic.Methods：Throughmakingmyelosuppressionanimalmodelofkunmingmicewith60Co-γrays，observethedifferenteffectofregulatingtheexpressionlevelsofperipheralhemogram，GM-CSF，EPO，andTPOinserumandCD34+inmarrowaboutChaihushengxueformula(self-protocoland)andXiaoyaopulvisonthebasisofthehemopoieticmethodofregulatingliver，Zhigancaodecoctiononthebasisofthehemopoieticmethodoftonifyingheart，Guipidecoctiononthebasisofthehemopoieticmethodsoftonifyingspleen，ZuoguipillandYouguipillonthebasisofthehemopoieticmethodoftonifyingkidney.Results：Comparedwithmodelcontrolgroup，eachmedicinegroupcouldimprovetheexpressionlevelofperipheralhemogramtodifferentdegrees，butZuoguipillandChaihushengxueformulacouldimprovetheexpressionlevelofHGB，RBCobviously（P＜0.05）andYouguipillcouldimprovetheexpressionlevelofRBC，WBCobviously（P＜0.05）.Comparedwithmodelcontrolgroup，eachmedicinegroupcouldimprovetheexpressionlevelofTPO，EPO，GM-CSFtodifferentdegrees（P＜0.05），butZuoguipill，ZhigancaodecoctionandChaihushengxueformulaweremoreadvantageouscomparedwithYouguipill，GuipidecoctionandXiaoyaopulvis（P＜0.05）.4Comparedwithmodelcontrolgroup，eachmedicinegroupcouldimprovethecontentofCD34+inmarrowtodifferentdegrees.ZuoguipillandXiaoyaopulvisweremoreadvantageousthanothergroups（P＜0.05）.Intermsofimpactonbonemarrowtissuepathologychange，eachmedicinegroupshowsthatbonemarrownucleatedcellsandmegakaryocytewereincreasedsignificantlyandbloodsinusexpansionwasnotobviouswhichrevealsthateachmedicinegroupcouldpromotebonemarrowtissue’srestor