少 壮 老不同年龄段肾阳虚体质基因表达谱的异同分析-similarities and differences of gene expression profiles of kidney yang deficiency in different age groups of shaozhuang and laozi.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 3 Abstract Objective: To reveal gene expression characteristics in different age groups of kidney yang deficiency through analysis of gene expression in different age groups of kidney yang deficiency, and to investigate gene regulation mode of kidney yang prevail and ail in young, mature, and old aged groups. Methods: According to kidney yang deficient constitution criteria of constitution classification criteria by China Association of Chinese Medicine, we had revised and improved these criteria for subject enrollment as inclusion criteria. Screening included cases strictly in accordance with inclusion criteria of kidney yang deficient constitution. The included cases were divided into three groups: young group (0-10 years old), mature group (29-39 years old) and old aged group (60-70 years old). The difference of gene expression profiles in different age groups of kidney yang deficient constitution was studied by technology of microarray. Screening differentially expressed genes, GO classification, molecular pathway analysis were all applied here to explore gene regulation mode of kidney yang prevail and ail in young, mature, and old aged groups. Results: Study of ancient and modern literatures had confirmed that constitution was extendedly applied and had significance in disease prevention and health care of Traditional Chinese Medicine Assessment questionnaire for kidney yang deficient constitution with 22 questions was developed for kidney yang deficient constitution diagnosis. 267 rows of differentially expressed gene in kidney yang deficient children were obtained from analysis of differentially expressed gene (P0.01), mainly involving hematopoietic system related genes; 344 rows of differentially expressed gene in kidney yang deficient mature group were obtained (P0.01), mainly involving cell growth and proliferation; 19 rows of differentially expressed gene in kidney yang deficient old aged group were gained (P0.01),