小鼠牙胚在肾包膜及口腔颊黏膜下成牙能力的对比分析-comparative analysis of tooth forming ability of mouse tooth embryo under renal capsule and buccal mucosa.docxVIP

4.移植物硬度和弹性模量结果显示：在釉质的硬度比较上，仅口腔黏膜4周组的釉质硬度比成年鼠牙低，差异具有统计学意义（P＜0.05），而其他组间两两比较的差别均无统计学意义（P＞0.05）。在釉质的弹性模量比较上，肾包膜下3周后的移植物釉质弹性模量比成年鼠牙略低，但差别无统计学意义（P＞0.05）；肾包膜下4周和口腔黏膜下4周后的移植物釉质弹性模量均低于成年鼠牙组和肾包膜3周的釉质弹性模量，差异具有显著的统计学意义（P＜0.01），但是这两组之间的差异无统计学意义（P＞0.05）。各组间牙本质的硬度及弹性模量无显著性差异（P＞0.05）。5.拉曼光谱分析结果显示：各组的拉曼光谱趋势基本一致，在961cm-1处有最大峰值，但是在2947cm-1处，口腔黏膜3周组有明显的峰值，远高于其他各组。结论1.对于ED14.5的牙胚来说，相对口腔黏膜环境而言，肾包膜下环境培育3~4周更能发育成相对完整的牙齿。2.口腔黏膜环境所形成牙齿的诸多性状与肾包膜环境下发育的牙齿相比虽有差异，但其对牙胚的生长发育还是具有一定的影响。关键词组织工程；牙胚；肾包膜；口腔黏膜；移植BackgroundandobjectiveAbstractInrecentyears,basedonthetheoryoftoothdevelopmentandstemcell,toothtissueengineeringandtoothregenerationbecomeahotpointinoralregenerativemedicine.However,thesuitableculturemodelofathree-dimensionaltoothgermstillremainselusive.Inthisstudy,weaimtocomparetwoenvironments,thesubrenalcapsuleandoralsubmucosa,forproducingwell-formedteethfrommousetoothgerms.MethodsFiftyICRmice(thirtywerefemale,twentyweremale,aboutthirtygramforeachmouse)weredevidedintotwogroupsinrandom:1.Experimentalgroup:GroupA-transplantedED14.5toothgermsintorenalsubcapsular,eachmousecanbetransplantedtwelvetoothgermsandtotallywerethreemiceandthirty-sixtoothgerms.GroupB-transplantedED14.5toothgermsintooralsubmucosalayer,eachmousecanbetransplantedtwotoothgermsandtotallywereeighteenmiceandthirty-sixtoothgerms.2.Normalcontrolgroup:GroupC-tookaembryonicday14.5pregnantmouse,didn’tdoanyprocessing,inordertomakeitsnaturalparturition.Atintervalsof3,4,8weeksaftertransplantation,thehostmiceweresacrificed,kidneysandoralmucousmembranesweredissectedtoobtaintheteeth.Andthenthetransplantedexplantswereevaluatedwithhistologicalstructures,hardnessandelasticmodulustest,andchemicalcompositions.ResultsMorphologyobservationofthegraft:themorphologyofthecrownwasquiteascontemporaneousmolaringroupA,inwhichithadobviouscusps.AstothegroupB,thecrownwassmaller,andtheshapeofthecuspswerenotsignificant,inallstudiestodate,itwasflatattheboredofcrownandroot,andtherewasnosignsofroots’development.HEstainingshowedthenormaltoothstructureinboththreegro