胃癌浸润转移相关质膜蛋白的鉴定及cdh4r-cadherin的功能学研究-identification of plasma membrane proteins related to invasion and metastasis of gastric cancer and functional study of cdh4r - cadherin.docxVIP

万方数据 万方数据 目 录 缩写词表································································································1 中文摘要································································································2 英文摘要································································································3 引 言································································································4 材料和方法·····························································································6 一、细胞株和菌株 ···················································································6 二、主要质粒 ·························································································6 三、主要仪器 ·························································································6 四、主要试剂 ·························································································7 五、常用溶液配制 ···················································································7 六、实验方法 ·························································································8 结 果······························································································ 21 一、胃癌细胞株 BGC-823 细胞活体荧光标记结果 ······································ 21 二、胃癌细胞浸润转移相关质膜蛋白的鉴定结果 ······································· 21 三、CDH4 的基因扩增片段 23 四、重组过表达质粒的连接酶切鉴定 ······················································· 23 五、CDH4 在组织及细胞水平中的实时定量 PCR 结果 ································ 24 六、HSP90、AIFM1 及 S100A9 在细胞水平中的实时定量 PCR 结果 ············· 25 七、Western blotting 检测 R-Cadherin 蛋白相关结果 ···································· 26 八、MTT 检测 CDH4 对胃癌细胞增殖的影响 ············································ 27 九、划痕实验检测转染前后 BGC-823 细胞的细胞迁移的差异 ······················ 28 十、Transwell 小室检测 CDH4 基因对胃癌细胞迁移侵袭能力的影响 ············· 28 讨 论·····················································································