辛伐他汀促进慢性肾病大鼠骨折愈合的初步分析-preliminary analysis of simvastatin promoting fracture healing in rats with chronic kidney disease.docxVIP

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辛伐他汀促进慢性肾病大鼠骨折愈合的初步分析-preliminary analysis of simvastatin promoting fracture healing in rats with chronic kidney disease.docx

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辛伐他汀促进慢性肾病大鼠骨折愈合的初步分析-preliminary analysis of simvastatin promoting fracture healing in rats with chronic kidney disease

PAGE PAGE IV Preliminary research of enhanced bone union by Simvastatin in rats with chronic kidney disease Abstract Preliminary research of enhanced bone union by Simvastatin in rats with chronic kidney disease Abstract Objective: To investigate the effects of simvastatin on the early period of fracture healing in rats with chronic kidney disease. Methods: Forty five SD rats at two months of age were divided into three groups: Normal+Vehicle, CKD+Vehicle and CKD+SVS. Rats in CKD+Vehicle and CKD+SVS groups were freely fed an adenine diet (0.75 g adenine in 100 g normal diet) for four weeks to establish CKD model. After 4 weeks of the adenine diet, blood sample were collected by jugular vein puncture for the test of serum level of calcium, phosphorus, SCr and BUN to confirm that the chronic kidney disease was established successfully, then the right midshaft tibiae osteotomy was performed and fixed with intramedullary wires. Treatment was begun at the second day after osteotomy and continued until sacrifice at 4 weeks post-fracture with saline vehicle and simvastatin at 80mg/kg/day by oral gavage in CKD+SVS group. 4 weeks after osteotomy, rats were killed by exsanguinations, the right tibiae were harvested for evaluation by micro CT, biomechanical test, and histomorphometry . Results: After 4 weeks of adenine diet, The levels of SCr , BUN and phosphorus of rats were significantly increased . The kidneys were swelling and pale, corresponding with the pathological change in CKD rats. Biomechanical test：Maximum load in CKD+ Vehicle group decreased 47.32% than that in Normal+Vehicle group（P0.05）；Maximum load in CKD+SVS group increased 49.45% than that in CKD+Vehicle group（P0.05）。Stiffness in CKD+ Vehicle group decreased 51.27% than that in Normal+ Vehicle group（P0.05）；Stiffness in CKD+SVS group increased 77.57% than that in CKD+Vehicle group（P0.05）。Energy uptake in CKD+Vehicle group decreased 46.84% than that in Normal+Vehicle group ；Energy