CD164对胶质瘤细胞生长影响研究.docVIP

CD164对胶质瘤细胞生长影响研究 　　摘要：目的 研究CD164在胶质瘤组织及细胞系中的表达，以及对体外胶质瘤细胞增殖的作用。方法 采用qRT-PCR分析CD164在45例胶质瘤组织和正常脑组织中的表达，利用western blot检测CD164在人胶质瘤细胞株SF295、SHG-44、U87和U251以及人正常星形胶质细胞系NHA中的表达；构建沉默CD164的慢病毒载体，并将其感染至胶质瘤细胞株U251，CCK-8法检测细胞增殖；western blot法检测感染CD164 shRNA后对U251细胞中PTEN蛋白表达的影响。结果 CD164在胶质瘤组织的表达较正常脑组织显著上调，差异有统计学（P0.001）；与NHA细胞相比较，CD164在人胶质瘤细胞系SF295、SHG-44、U87和U251细胞中的表达量明显增加，具有明显的统计学差异性（P0.001）；U251细胞感染CD164 shRNA后，细胞存活率较阴性对照组下降，两组结果存在明显的统计学差异（P0.001）；感染CD164 shRNA至U251细胞后PTEN蛋白表达水平明显升高。结论 CD164可能通过调控PTEN的表达从而影响胶质瘤细胞增殖。 　　关键词：胶质瘤；CD164；PTEN；增殖 　　中图分类号：R739.41 文献标识码：A 文章编号：1006-1959（2017）22-0029-04 　　Abstract：Objective To investigate the expression of CD164 in glioma tissues and cell lines，and the effects on glioma cell proliferation in vitro. Methods The expression of qRT-PCR of CD164 in 45 cases of glioma tissues and normal brain tissues，using western blot to detect CD164 in the human glioma cell lines SF295，SHG-44，U87 and U251 and the expression of the normal astrocyte cell line NHA；lentiviral vector construction of CD164 silencing，and the infection to U251 glioma cell line，cell proliferation was detected by CCK-8；CD164 shRNA western blot infection detection method of U251 cells PTEN protein expression.Results The expression of CD164 in glioma compared with normal brain tissue the organization was significantly increased，the difference was statistically（P0.001）；compared with NHA cells，CD164 in human glioma cell lines SF295，SHG-44， expression of U87 and U251 cells increased significantly，a statistically significant difference（P0.001）；U251 cells infected with CD164 shRNA，the cell survival rate decreased compared with negative control group，there were statistically significant differences between the results of the two groups（P0.001）；the expression level of PTEN protein was significantly increased CD164 shRNA infection to U251 cells.Conclusion CD164 may affect the proliferation of glioma cells by expression the regulation of PTEN. 　　Key words：Glioma；CD164；PTEN；Proliferati