CD40单克隆抗体枝联聚乳酸体外扩增树突状细胞应用.docVIP

CD40单克隆抗体枝联聚乳酸体外扩增树突状细胞应用 　　【摘要】 目的 研究聚乳酸枝联CD40单克隆抗体在树突状细胞（DC）培养中的作用， 从而对三维立体培养有深入的认识， 为体外扩增树突状细胞提供新思路。方法 利用鼠抗人CD40单克隆抗体对DC的诱导效应， 观察聚乳酸枝联CD40单克隆抗体诱导的树突状细胞数量及生物学功能变化。结果 与对照组普通悬液培养体系树突状细胞数量（5.43±33.31）×108比较， 实验组三维立体培养系统树突状细胞数量（8.22±25.25）×108扩增（P0.05）。实验组三维立体培养系统在6、9 d促进人树突状细胞分泌白细胞介素-12b（IL-12b）量高于对照组（P0.05）。与对照组比较， 实验组更加能促进人树突状细胞的成熟， 促进其表面共刺激分子表达水平上调。结论 通过CD40单克隆抗体与聚乳酸支架枝联建立的三维立体培养系统， 有利于树突状细胞数量的扩增及其功能的增进。 　　【关键词】 CD40单克隆抗体；聚乳酸支架；三维立体培养系统；树突状细胞 　　DOI：10.14163/j.cnki.11-5547/r.2016.25.198 　　【Abstract】 Objective To research effect by polylactic acid scaffold combined with CD40 monoclonal antibody in dendritic cell （DC） culture， in order to get further knowledge of three-dimensional culture， and to provide new thoughts for dendritic cell expansion in vitro. Methods Dendritic cell count and changes of biological function in olylactic acid scaffold combined with CD40 monoclonal antibody induction were observed in accordance with inductive effect by mouse anti-human CD40 monoclonal antibody on DC. Results Comparing with （5.43±33.31）×108 of dendritic cell count in control group by common suspension culture system， experimental group had expansion of dendritic cell count as （8.22±25.25）×108 by three-dimensional culture system （P0.05）. Experimental group had higher interleukin-12b （IL-12b） level in dendritic cell accelerated by three-dimensional culture system in 6 and 9 d than control group （P0.05）. Comparing with control group， experimental group accelerated dendritic cell maturation and increased surface costimulatory molecules level. Conclusion Three-dimensional culture system established by combination of CD40 monoclonal antibody and bolylactic acid scaffold is helpful for dendritic cell expansion and function enhancement. 　　【Key words】 CD40 monoclonal antibody； Polylactic acid scaffold； Three-dimensional culture system； Dendritic cell 　　近年来， 组织工程方面的研究得到了突飞猛进的发展， 聚乳酸由丙交酯开环聚合制备， 具有无毒、可靠的生物安全性， 被美国FDA批准用于临床， 是迄今研究最广泛、应用最多的可降解生物材料[1]。DC在外周血中含量极低， 在抗肿瘤免疫中发挥重要作用[2]。因此