CD44v3和CD44v5在口腔黏液表皮样癌中表达及临床意义.docVIP

CD44v3和CD44v5在口腔黏液表皮样癌中表达及临床意义 　　[摘要]目的：通过检测细胞表面黏附分子CD44v3和CD44v5在口腔黏液表皮样癌（Oral mucoepidermoid carcinoma，OMC）中的表达，探讨二者与OMC的发生、病理分级和临床分期的关系。方法：利用免疫组织化学方法检测40例OMC石蜡标本中CD44v3和CD44v5蛋白表达，并且分组进行统计学分析。结果：CD44v3和CD44v5在OMC中表达率与正常对照组相比有显著升高（P0.05）。结论：在OMC的淋巴结转移中，CD44v3和CD44v5可能起着重要作用，CD44v3、CD44v5可能成为了解OMC病变发生发展生物学行为及淋巴结转移重要的生物学指标之一。 　　[关键词]口腔黏液表皮样癌；CD44v3；CD44v5；免疫组化 　　[中图分类号]R739.85 [文献标志码]A [文章编号]1008-6455（2015）04-0032-03 　　Expression and clinical significance of CD44v3 and CD44v5 in oral mucoepidermoid carcinoma 　　MA Zuo1，HU Shan-shan2，ZHOU Yang1，SUN Hao-liang1，LI Hui1，LI Qiao-ling1 　　（1.Department of Stomatology，2.Department of Ophthalmology，Red Flag Hospital，Mudanjiang Medical University，Mudanjiang 157011，Heilongjiang，China） 　　Abstract： Objective To detect the expression of the cell surface adhesion molecule CD44v3 and CD44v5 in oral mucoepidermoid carcinoma（OMC），and to explore the relationship between the two and the occurrence，pathological grade and clinical stage of OMC. Methods Immunohistochemical staining was used to detect the exprssion of CD44v3 and CD44v5 protein in 40 cases of OMC in paraffin embedded specimens， and were statistically analyzed. Results CD44v3 and CD44v5 expression rates in OMC compared with the control group was significantly higher （P0.05）. Conclusion OMC in the lymph node metastasis，CD44v3 and CD44v5 may play an important role，CD44v3，CD44v5 may be one of the important indexesto understand the transfer of OMC lesion development and the biological behavior of lymph node. 　　Key words：oralmucoepidermoid carcinoma；CD44v3；CD44v5；immunohistochemistry 　　口腔黏液表皮样癌（Oral mucoepidermoid carcinoma，OMC）是临床十分常见的口腔恶性肿瘤之一[1]，临床研究表明其细胞学行为与肿瘤的生物学行为高度相似。临床上将这一类广泛分布的细胞表面黏附分子均称为CD44，由于CD44亚型是拼接有外显子的变异亚型码，因此研究人员将CD44家族中含有变异型外显子v3和v5编码序列的CD44分子依次命名为CD44v3和CD44v5，且其多在上皮源性或肿瘤细胞中表达，故临床报道较多，报道多集中讨论于其与胆囊癌、肾癌、非小细胞肺癌、肠癌以及口腔鳞状细胞癌等癌症的相关性[2-3]。通过以CD44v为关键词搜索国内外相关文献，发现其中关于CD44v3和CD44v5与口腔黏液表皮样癌的报道十分罕见，但相关学者的研究表明CD44v3和CD44v5对口腔黏液表皮样癌的诊断和治疗具有一定的临床价值[4]。故本课题应用免疫组织化学方法