miR130a3p下调CYLD表达对乳腺癌MCF7细胞功能影响.docVIP

miR130a3p下调CYLD表达对乳腺癌MCF7细胞功能影响 　　摘 要 目的：探讨miR-130a-3p对人乳腺癌MCF-7细胞功能的影响及其可能的靶向基因。方法：应用脂质体介导方法将miR-130a-3p模拟物（实验组）或对照模拟物（对照组）转染MCF-7细胞，分别采用MTT方法和细胞划痕实验检测细胞增殖和迁移能力变化，印迹法检测细胞中miR-130a-3p靶基因圆柱瘤基因（CYLD）的表达。结果：与对照组比较，实验组MCF-7细胞的增殖和迁移能力增强（P＜0.05），细胞中CYLD蛋白表达水平明显下调（P＜0.05）。结论：miR-130a-3p可能通过靶向调节CYLD蛋白表达增强人乳腺癌MCF-7细胞的增殖和迁移。 　　关键词乳腺肿瘤圆柱瘤基因miR-130a-3pMCF-7细胞 　　中图分类号：R737.9文献标识码：A文章编号：1006-1533（2014）12-0003-04 　　The effect of miR-130a-3p on function of human breast cancer cell line MCF-7 by down-regulating the expression of CYLD 　　LI Dengfeng, WEI Chuankui, SONG Hongming, LI Xiaoyu, LUO Qifeng, CHEN Lei，HUANG Yixiang, JIAN Wei, FANG Lin 　　（Department of Breast and Thyroid Surgery, Tenth People’s Hospital, Tongji University, Shanghai 200072, China） 　　ABSTRACT Objective: To investigate the effect of miR-130a-3p on the function of human breast cancer cell line MCF-7, and to explore its possible target gene. Methods: miR-130a-3p mimics or its negative control (miR-130a-3p mimics-NC) was transfected into MCF-7 cells by lipofectamine. The capacities of proliferation and migration of MCF-7 cells were evaluated by MTT and Scratch assay, respectively. The expression of CYLD which was predicted as a target gene in MCF-7 cells was determined by Western blotting. Results: The capacities of proliferation and migration in MCF-7 cells transfected with miR-130a-3p mimics were significantly promoted and the expression level of CYLD was down-regulated when compared with those in MCF-7 cells transfected with miR-130a-3p mimics-NC（P＜0.05). Conclusion: The miR-130a-3p expression can promote the proliferation and migration of human breast cancer line MCF-7 by down-regulating the expression of CYLD. 　　KEY WORDSbreast neoplasms; cylindromatosis; mir-130a-3p;MCF-7 cell 　　乳腺癌是女性常见的恶性肿瘤，也是女性癌症死亡的主要原因之一，全球每年死亡病例超过50万[1]。虽然,近年来随着乳腺癌筛查工作的开展，早期乳腺癌确诊病例比例增加，但晚期死亡率并无明显下降，因此，寻找乳腺癌特异标志物对临床诊断意义重大。已有研究提出，将微小RNA（miRNA）作为肿瘤分子标志，可用于辅助癌症的诊断和预后评估[2-3]。目前，已有研究显示miR-130a-3p在肝胰岛素敏感性和肝脂肪变中发挥作用[4]，并且在尿道膀胱癌中与肿瘤的发生和发