OPN与VEGF在子宫腺肌病中表达及意义.docVIP

OPN与VEGF在子宫腺肌病中表达及意义 　　【摘要】 目的：检测骨桥蛋白（osteopontin，OPN）及血管内皮生长因子（vascular endothelial growth factor，VEGF）在子宫腺肌病病灶、子宫腺肌病在位内膜、正常子宫肌层及正常子宫内膜中的表达，从而探讨OPN、VEGF在子宫腺肌病发生与发展中的作用及两者的相关性。方法：采用免疫组织化学方法（S-P法）检测50例子宫腺肌病患者子宫腺肌病病灶、在位内膜及50例子宫肌瘤患者正常子宫肌层及正常子宫内膜中的OPN与VEGF的PU值。比较两组间OPN与VEGF表达水平差异。结果：OPN、VEGF在腺肌病在位内膜分泌期中的PU值高于增生期（P0.05）；OPN、VEGF在腺肌病在位内膜增生期与分泌期的PU值均高于在正常内膜增生期与分泌期的PU值（P0.05）。OPN、VEGF在子宫腺肌病中的表达经Pearson积差相关分析，无相关关系（r=0.014，P=0.920）。结论：OPN与VEGF在子宫腺肌病异位内膜及在位内膜中的过度表达，两者可能参与了子宫腺肌病的发生与发展过程。OPN与VEGF两者在子宫腺肌病中的表达无相关性，表明两者在子宫腺肌病的发生与发展过程中可能各自独立起到调控作用。 　　【关键词】 子宫腺肌病； 骨桥蛋白； 血管内皮生长因子 　　【Abstract】 Objective：To detect the expression of osteopontin（OPN） and vascular endothelial growth factor（VEGF） in adenomyosis focus，eutopic endometrium of adenomyosis，normal myometrium and normal endometrium to discuss the effect of OPN and VEGF in the occurrence and development of adenomyosis and the correlation between OPN and VEGF.Method：Immunohistochemical method（S-P method） was used to detect PU value of OPN and VEGF in adenomyosis focus，eutopic endometrium of adenomyosis of 50 patients with adenomyosis，and normal myometrium and normal endometrium of 50 patients with uterine fibroid.The differences regarding OPN and VEGF expression levels between two groups were compared.Result：PU value of OPN and VEGF in the proliferative and secretory phase of eutopic endometrium of adenomyosis was higher than that in normal proliferative phase，and the difference was significant （P0.05）.PU values of OPN and VEGF in the proliferative and secretory phase of eutopic endometrium of adenomyosis were higher than those in the normal endometrial proliferative and secretory phase，there was a significant difference between two groups（P0.05）.The expression of OPN and VEGF in adenomyosis was analyzed by Pearson’s product-moment correlation analysis.There was no correlation （r=0.014，P=0.920）.Conclusion：The overexpression of OPN and VEGF in ectopic endometrium and eutopic endometrium of adenomyosis may be involved in the occu