N乙酰半胱氨酸对大鼠慢性支气管炎与肺气肿形成影响实验研究.docVIP

N乙酰半胱氨酸对大鼠慢性支气管炎与肺气肿形成影响实验研究 　　【摘要】 目的 研究抗氧化剂N-乙酰半胱氨酸(NAC)对大鼠慢性支气管炎（CB，chronic bronohitis）与肺气肿形成的影响。方法 24只健康雄性Wistar大鼠用完全随机设计随机分为4组，A组(假吸烟组)(n=6)、B组(慢性支气管炎并肺气肿组)(n=6)、C组(NAC预防组)(n=6)、D组(NAC治疗组)(n=6)，B、C及D组采用两次气管内注入脂多糖(LPS)及烟熏4周法制备慢性支气管炎并肺气肿大鼠模型，C组均在烟熏及气管内注入脂多糖前每天给予NAC(50 mg/只)灌胃，D组在第15~28天烟熏前每天给予NAC(50 mg/只)灌胃。各组对肺组织行HE染色观察肺泡面积改变。结果 B组小气道均存在纤毛损伤、上皮细胞变性坏死糜烂脱落、黏液杯状细胞增生、气道管壁黏膜充血水肿、管壁炎性细胞浸润(淋巴细胞、中性粒细胞、单核细胞)(P0．05)，说明C组肺气肿不明显,与A组比较D组大鼠仍有一定程度的肺气肿(P0．05)。结论 NAC抗氧化可抑制由吸烟及脂多糖诱导的大鼠慢性支气管炎并肺气肿的小气道炎症、拮抗小气道损伤及防治肺气肿形成。 　　【关键词】N-乙酰半胱氨酸；大鼠；慢性支气管炎；肺气肿 　　 　　The experimental study on effect of antioxidant on formation of chronic bronchitis and emphysema in rats 　　 　　LIU Ji-feng,ZHONG Xiao-ning,HE Zhi-yi,et al.Department of Respiratory Diseases,the First Affliated Hospital of Guangxi Medical University,Nanning 530021,China 　　 　　【Abstract】 Objective The purpose of the present study was to investigate the effect of N-acetylcysteine(NAC)on formation of chronic bronchitis and emphysema in rats.Methods 24 healthy Wistarmale rats were divided into 4 groups randomly.Group B(CB and emphysema),Group C(NAC prevention)and Group D(NAC treatment)were instilled intratracheally with LPS twice and exposed to cigarette smoking 4 weeks to induce CB and emphysema model.Group C were administrated with NAC 50 mg every rat before given LPS and smoking.Group D were administrated with NAC 50 mg every rat from the 15th to the 28th day before given smoking.The morphology changes of alveoli area have been observed.Results Compared with group A,there were injury of cilium,necrosis and erosion of epithelium,proliferation of goblet cells,mucous hyperemia and edema,inflammatory cells infiltration in the tubal walls(including lympholeukocyte,neutrophil and monocyte)in group B,and meanwhile the average area of alveolus in group B increased(P0．05).group D with the treatment of NAC had emphysema to some extentConclusion NAC can attenuate the inflammation and injury in small airways of chronic bronchitis and emph