siRNA干扰FOXF2对人宫颈癌SiHa细胞体外转移影响.docVIP

siRNA干扰FOXF2对人宫颈癌SiHa细胞体外转移影响 　　[摘要]目的 研究siRNA干扰叉头框转录因子F2（FOXF2）对人宫颈癌SiHa细胞体外转移能力的影响及其作用机制。方法 采用RT-PCR、Western blot分别检测siRNA-FOXF2转染前后，SiHa细胞中FOXF2 mRNA、蛋白的表达变化；同时用划痕试验和Transwell侵袭试验分别检测siRNA-FOXF2转染前后细胞的迁移和侵袭能力，CCK8检测siRNA-FOXF2转染前后细胞的增殖能力。结果 SiHa细胞内FOXF2敲除后，RT-PCR、WB检测显示siRNA-FOXF2转染组FOXF2的蛋白、mRNA均明显下降；划痕试验和Transwell侵袭试验显示细胞侵袭、迁移能力增强；CCK8检测显示siRNA-FOXF2转染组促进细胞增殖能力（P0.01）。结论 siRNA干扰FOXF2促进SiHa细胞的体外转移能力及细胞增殖能力，进而促进宫颈癌的发生发展。 　　[关键词]宫颈癌；叉头框转录因子F2；SiHa细胞；细胞迁移；细胞侵袭；细胞增殖 　　[中图分类号] R737.3 [文献标识码] A [文章编号] 1674-4721（2017）03（c）-0004-04 　　[Abstract]Objective To explore the effect of siRNA silencing FOXF2 on invitro metastasis of human cervical carcinoma SiHa cells and its mechanism.Methods The expression of FOXF2 mRNA and protein in SiHa-siRNA-FOXF2 cells and negative control cells were detected by RT-PCR and Western blot respectively. The migration and invasion ability of cells were detected by wound-healing assay and Transwell assay before and after siRNA-FOXF2 transfection.CCK8 was used to detect the proliferation of cells before and after siRNA-FOXF2 transfection.Results The expression of FOXF2 mRNA and protein in siRNA-FOXF2 transfected group was significantly decreased after FOXF2 knockout in SiHa cells by RT-PCR and WB detection；the invasion and migration were enhanced by wound-healing assay and transwell assay.After transfection of siRNA-FOXF2 into cervical cancer SiHa cell lines，the capability of cell proliferation was significantly enhanced （P0.01）.Conclusion siRNA-FOXF2 can promote the invitro metastasis and proliferation of SiHa cells，and promote the development of cervical cancer. 　　[Key words]Cervical cancer；FOXF2；SiHa cells；Cell invasion；Cell migration；Cell proliferation 　　宫颈癌是临床常见的妇科恶性肿瘤，其发病率居女性恶性肿瘤第3位，死亡率居第4位[1]，且发病趋势呈低龄化[2]。目前主要的治疗方法是宫颈癌根治术和放化疗，其对早期患者的治愈率高达80%～95%，但对于晚期和复发转移的患者仍无令人满意的治疗方法。因此，从分子水平研究宫颈癌的发病和预防机制，采取针对性的靶向治疗迫在眉睫。叉头框转录因子F2（forkhead boxF2，FOXF2）蛋白为FOX转录因子家族中的成员之一，主要存在于泌尿道、呼吸道和消化道等系统器官的邻近上皮间质细胞，其参与细胞外基质（extracellular matrix，ECM）合成[3]、胚胎及组织发