P27kipl和ki67蛋白在病理性瘢痕组织中表达和意义.docVIP

P27kipl和ki67蛋白在病理性瘢痕组织中表达和意义 　　[摘要]目的：研究病理性瘢痕组织中P27kipl和ki-67蛋白的表达情况及其相互关系，探讨他们在瘢痕形成中的作用及机制。方法：应用免疫组化SP法检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中P27kipl、ki-67蛋白的表达并进行统计学分析。结果：病理性瘢痕组织中P27kipl蛋白表达显著低于正常皮肤、成熟瘢痕（P=0.036）; ki-67蛋白在病理性瘢痕组织中的表达显著高于正常皮肤、成熟瘢痕（P＜0.000）；病理性瘢痕组织中P27kipl的表达与ki-67蛋白表达呈负相关（P＜0.000）。结论：P27kipl蛋白表达的下降及ki-67的表达升高可能与病理性瘢痕的形成有关。 　　[关键词]P27kipl蛋白；ki-67蛋白；增生性瘢痕；瘢痕疙瘩 　　[中图分类号]Q813 [文献标识码]A[文章编号]1008-6455（2008）10-1477-03 　　 　　Expression and significance of P27kipl and ki-67 protein in pathological scar 　　WU Wen-yi,ZHU Shi-ze,WU Rui-lan,ZOU Cheng-hao,WANG Zhao-yang,HUANG Zhong-xin 　　(Departent ofPlastic Surgery,the Second Affiliated Hospital of Fujian Medical University,Quanzhou362000,Fujian,China) 　　 　　Abstract:ObjectiveTo study the expression of P27kipl and ki-67 in the pathological scars and their reaction-ship and to investigate its role and its possible mechanism in the pathogenesis of abnormal scars.MethodsImmunohistochemiscal technique was performered to detect the expression and distribution of P27kipl and ki-67 protein in hypertrophic scar (42cases), keloid (18cases), normal scar (40 cases) and normal skin (50cases),statistics was used to analyze the data.ResultsThe positive rate of P27kipl and ki-67 protein expression were remarkably significant in comparison between normal scar and abnormal scar(P0.05). In pathological scar the protein of P27kipl and ki-67 showed a strong inverse correlation (P0.000). ConclusionLow expression of P27kipl and high expression of ki-67 may play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar. 　　Key words: P27kipl protein；ki-67 protein；hypertrophic scar；keloid 　　 　　增生性瘢痕和瘢痕疙瘩是临床上最常见的病理性瘢痕，其形成机制仍不十分清楚。近年来研究表明，病理性瘢痕的形成是以细胞的增生和凋亡失衡为基础的[1-2]。深入研究细胞增殖和凋亡在病理性瘢痕形成中的作用，将有利于揭示其发病机制。P27kipl 是一种广谱的周期蛋白依赖性激酶抑制剂，是G1/S期限制的重要调控分子。ki-67是被公认的反映细胞增殖活性的标志物。我们应用免疫组化方法，检测了增生性瘢痕、瘢痕疙瘩组织中P27kipl、 ki-67蛋白的表达情况，并与成熟瘢痕；正常皮肤作了比较，初步探讨P27kipl、ki-67的表达与病理性瘢痕形成的关系，为瘢痕的防治提拱一定实验依据。 　　 　　1材料和方法 　　1.1 组