Galectin―9蛋白过表达对卵巢癌细胞增殖迁移及凋亡影响.docVIP

Galectin―9蛋白过表达对卵巢癌细胞增殖迁移及凋亡影响 　　[摘要] 目的 探?半乳糖凝集素-9（Galectin-9）蛋白过表达对卵巢癌细胞增殖、迁移和凋亡的影响。 方法 将卵巢癌细胞SKOV-3分为转染pcDNA3.1-Galectin-9组、pcDNA3.1组和空白对照组，通过Western blot检测Galectin-9蛋白的表达，CCK-8检测Galectin-9蛋白对SKOV-3增殖能力的影响，划痕试验验证Galectin-9对SKOV-3迁移能力的作用，Annexin-V-FITC/PI双染法检测Galectin-9蛋白对SKOV-3凋亡的影响。 结果 Western blot结果显示，转染pcDNA3.1-Galectin-9组Galectin-9蛋白表达明显高于空白对照组和pcDNA3.1组，差异均有高度统计学意义（均P 0.01）；CCK-8结果显示，转染pcDNA3.1-Galectin-9组增殖能力明显低于空白对照组和pcDNA3.1组，差异均有高度统计学意义（均P 0.01），Galectin-9能显著抑制细胞的增殖能力，并且抑制细胞的增殖能力随着时间的延长而增强；细胞划痕试验结果显示，转染pcDNA3.1-Galectin-9组的愈合率明显高于pcDNA3.1组和空白对照组，差异均有高度统计学意义（均P 0.01）；流式细胞试验结果显示，转染pcDNA3.1-Galectin-9组的凋亡率明显高于pcDNA3.1组和空白对照组，差异均有高度统计学意义（均P 0.01）。 结论 Galectin-9的过表达能够抑制卵巢癌细胞株的增殖和迁移能力，并促进其凋亡。 　　[关键词] 半乳糖凝集素-9；卵巢癌；细胞增殖；细胞迁移；细胞凋亡 　　[中图分类号] R737.31 [文献标识码] A [文章编号] 1673-7210（2018）01（c）-0013-04 　　[Abstract] Objective To investigate the effects of overexpression of galectin-9 on the proliferation， migration and apoptosis of ovarian cancer cells. Methods The ovarian cancer cells SKOV-3 were divided into pcDNA3.1-galectin-9 transfection group， pcDNA3.1 group and blank control group， Western blot was used to detect the expression of galectin-9 protein， CCK-8 was used to detect the effects of galectin-9 protein for multiplication capacity of SKOV-3， scratch test was used to verify the effects of galectin-9 protein for transfer ability of SKOV-3， Annexin-V-FITC/PI double staining method was used to detect the effects of galectin-9 protein for apoptosis of SKOV-3. Results The results of Western blot showed that the expression of galectin-9 protein in pcDNA3.1-galectin-9 transfection group was significantly higher than those in blank control group and pcDNA3.1 group， the differences were all highly statistically significant （all P 　　[Key words] Galectin-9； Ovarian cancer； Cell proliferation； Cell migration； Cell apoptosis 　　卵巢癌为妇科常见的恶性肿瘤之一，具有侵袭性强、恶性程度高、转移快、病死率高和复发率高等特点[1]，半乳糖凝集素-9（Galectin-9）是半乳糖凝集素家族成员之一，具有嗜酸性粒细胞趋化作用及抗肿瘤功能，在多种恶性肿瘤组织中呈低表达。文献报道，通过免疫组织化学发现，Galec