RtaIgG在珠海地区鼻咽癌诊断中应用价值.docVIP

RtaIgG在珠海地区鼻咽癌诊断中应用价值 　　摘要：目的探讨EB病毒的Rta蛋白抗体IgG（Rta/IgG）在珠海地区鼻咽癌(nasopharyngeal carcinoma，NPC)患者的临床诊断价值。方法收集75例病理证实未经治疗的NPC患者血清、100例健康成人血清及肝癌、胃癌、肺癌、淋巴瘤、宫颈癌血清各19例。检测①Rta/IgG在NPC、肝癌、胃癌、肺癌、淋巴瘤、宫颈癌、健康人群的表达水平；②Rta/IgG与鼻咽癌临床分期关系；③Rta/IgG、VCA/IgA、Rta/IgG联合VCA/IgA对鼻咽癌检测率。结果①NPC患者Rta/IgG阳性率远高于健康人水平，具有显著性差异（P0.05）。③Rta/IgG、VCA/IgA及VCA/IgA联合Rta/IgG检测NPC的敏感度分别为81．3％、89．3％、94.6％；特异度分别为93％、84％、80%。结论①Rta/IgG是NPC早期辅助诊断及筛查的可靠的血清学指标。②Rta/IgG的表达与NPC临床分期无关。③Rta/IgG联合VCA/IgA检测提高了NPC的筛检和诊断灵敏度。 　　关键词：EB病毒Rta/IgG；VCA/IgA；鼻咽癌；诊断 　　To Evaluate the Clinical Value of Epstein-Barr Virus (EBV)s Rta/IgG in the Diagnosis of Nasopharyngeal Carcinoma (NPC) Patients in Zhuhai 　　XU Qin,ZHENG Shui-hua,LIN Su-xiang 　　(Clinical Laboratory,The Peoples Second Hospital of Zhuhai City,Zhuhai 519000,Guangdong,China) 　　Abstract：ObjectiveTo evaluate the clinical value of Epstein-Barr virus (EBV) Rta/IgG in the diagnosis of nasopharyngeal carcinoma (NPC) patients in Zhuhai. Methods Rta/IgG and VCA/IgA was detected by enzyme-linkedimmunosorbent assay in 75 NPC patients,100 healthy adults and 60patients with different tumor.Results(1)Significant difference of Rta/IgG positive rate between NPC group and the healthy control group (P0.01) as well as between NPC group and other carcinoma groups(P0.01).(2)The expression levels of Rta/IgG in NPC patients serum were irrelative to the clinical stage of the tumor(P0.05).(3)The sensitivity rates were 81.3% ,88.9%, 94.6% for Rta/IgG ,VCA/IgA , Rta/IgG or VCA/IgA respectively, while the specificity rates were 94.6%,84%,80% respectively.ConclusionRta/IgGis a sensitive and specific serologic parameter for NPC diagnosis .but it is not a proper maker for evaluating clinical stage of NPC.The combined detection of Rta/IgG and VCA/IgA has referential value in diagnosing nasopharyngeal carcinoma patients in Zhuhai. 　　Key words：EBV Rta/IgG;VCA/IgA;Nasopharyngeal carcinoma(NPC);Diagnosis鼻咽癌（NPC)是我国华南地区的常见肿瘤，素有“广东瘤”之称。广东省每年新发病例约5000例。NPC是一种源于鼻咽部上皮组织的一种恶性程度较高的肿瘤。筛选特异性的NPC将有助于NPC早