TCT联合HPV E6E7 mRNA定量检测宫颈上皮内瘤变临床应用价值.docVIP

TCT联合HPV E6E7 mRNA定量检测宫颈上皮内瘤变临床应用价值 　　【摘要】 目的：探讨利用HPV E6/E7 mRNA检测技术检测妇科子宫颈上皮内瘤变的临床应用价值。方法：对69例TCT检查结果为宫颈上皮内瘤变的患者，应用HPV E6/E7 mRNA检测技术对感染HPV病毒中的癌基因片段E6、E7转录子做出定量检测。结果：ASC-H组E6/E7 mRNA转录阳性率、转录数分别为60.6%、（18 285.42±1487.71），均高于≤ASC-H组的33.3%、（2668.43±681.39），差异均有统计学意义（P0.05）；宫颈细胞学检查为宫颈癌的HPV E6/E7 mRNA转录数明显高于其他类型，差异有统计学意义（P0.05）。结论：宫颈上皮内瘤变与E6/E7 mRNA转录具有相关性，即转录数与病变的进展程度及细胞的恶性转化密切相关，在宫颈癌筛查中检测HPV E6/E7 mRNA的转录具有潜在价值。 　　【关键词】 TCT； HPV E6/E7 mRNA定量检测； 宫颈上皮内瘤变 　　【Abstract】 Objective：To discuss the clinical application value of quantitative detection the HPV E6/E7 mRNA in cervical intraepithelial neoplasia.Method：In 69 cases of TCT examination results for cervical intraepithelial neoplasia，use of HPV E6/E7 mRNA detection technology for the infection of HPV virus in the cancer gene fragment E6，E7 transcription to make quantitative detection.Result：ASC-H group HPV E6/ E7 mRNA transcription positive rate （60.6%） was higher than the≤ASC-H group （33.3%），ASC-H group HPV E6/E7 mRNA transcription number （18 285.42±1487.71） was higher than the ≤ASC-H group （2668.43±681.39），the differences were statistically significant（P0.05）.Cervical cytological examination for cervical cancer HPV E6/E7 mRNA transcription number was significantly higher than other types，the difference was statistically significant（P0.05）.Conclusion：The cervical intraepithelial neoplasia is closely related to the E6/E7 mRNA transcription，that is，and that means the HPV E6/E7 mRNA transcription affect the the progression of lesions and malignant transformation of cells，quantitative detection the HPV E6/E7 mRNA has potential clinical application value in the screening of cervical intraepithelial neoplasia. 　　【Key words】 TCT； Quantitative detection of HPV E6/E7 mRNA； Cervical intraepithelial neoplasias 　　First-author’s address：The First Affiliated Hospital of Baotou Medical College to Inner Mongolia University of Science Technology，Baotou 014010，China 　　doi：10.3969/j.issn.1674-4985.2016.36.029 　　研究?J为单一的宫颈细胞学检查不论是