一次递增负荷至力竭运动对经过训练男性田径运动员红细胞变 形性和eNOS影响.docVIP

一次递增负荷至力竭运动对经过训练的男性田径运动员红细胞变 形性和eNOS的影响 　　摘 要： 目的：探究长期运动训练对青年男性红细胞变形性与红细胞内皮型一氧化氮合酶（e NOS）的影响及可能机制；方法：训练组为20名男子专业运动员，对照组为20名男子大学生 。两组均在功率自行车上完成递增负荷力竭运动。运动前和运动后即刻采集静脉血，测定红 细胞变形指数、血浆NO含量、红细胞eNOS活性、红细胞eNOS蛋白总量及Ser1177-eNOS磷酸化 水平。结果：组间比较，运动前后，训练组上述指标均显著高于对照组。组内比较，运动前 后，对照组和训练组上述指标均显著降低，但训练组降低幅度明显低于对照组。结论：长期 运动训练可通过增加Ser1177-eNOS磷酸化水平上调红细胞eNOS-NO系统功能，提高红细胞变 形性；并可提高其对急性大负荷运动的应激能力。 　　关键词：递增负荷至力竭运动；田径运动员；红细胞变形性；内皮型一氧化 氮合酶 　　中图分类号：G804.7文献标识码：A文章编 号：1007-3612(2011)04-0054-03 　　Effects of a Single Bout of Incremental and Exhaustive Exerciseon Erythrocyte Deformability and eNOS in Training Male Elite Athletes 　　 　　LIU Li?xin 　　(Department of Physical Education，Northeast Petroleum Universi ty，Daqing 163318，Heilongjiang China) 　　Abstract: Objective: To explore the effects of exercise training on erythrocyte deformabil ity and erythrocyte endothelial nitric oxide synthase (eNOS) in male elite youngpeople. Methods: The volunteers of training group were 20 male athletes，and t he volunteers of control group were 20 male college students. All volunteers we re asked to do the incremental cycle test. Blood of elbow vein was collected be fore and after the incremental exercise. Then the erythrocyte deformability，pl asma nitric oxide (NO) content，activity of erythrocyte eNOS，amount of total er ythrocyte eNOS protein and level of phosphorylation states of eNOS were measured . Results: Before and after exercise，all parameters we have measured in traini ng group were notably higher than those in control group. Both in control groupand training group，all parameters we have measured were significantly depresse d after the incremental exercise. It is noteworthy that the change extent of th ese parameters in training group was significantly lower than that in control gr oup. Conclusion: Exercise training can improve erythrocyte deformability and ac tivate erythrocyte eNOS through upregulation of Ser1177?eNOS，which may also el evate resistance to high intensity exercise. 　　Key words: in