五种植物蛋白源替代鱼粉对花鲈血清生化指标转氨酶活性及抗氧化应激参数影响.docVIP

五种植物蛋白源替代鱼粉对花鲈血清生化指标、转氨酶活性及抗氧化应激参数的影响 　　摘要：选用480尾体重为（9.69±0.15） g的花鲈（Lateolabrax japonicus）随机分成6组，分别投喂含40%鱼粉的基础饲料（鱼粉组）和用23.30%豆粕、22.40%花生粕、23.82%棉粕、27.77%菜子粕、38.98%玉米DDGS蛋白替代16%鱼粉的5种替代饲料（替代组），饲养28 d后测定血清生化指标、转氨酶活性和抗氧化应激参数。结果表明，各替代组血清中血糖（GLU）、尿素氮（UN）和低密度脂蛋白胆固醇（LDL-C）水平与鱼粉组相比差异不显著（P0.05）。DDGS组血清总蛋白（TP）含量显著低于鱼粉组（P0.05），花生粕、棉粕、菜子粕和DDGS组血清谷丙转氨酶（ALT）活性显著降低（P0.05），菜子粕和DDGS组血清过氧化氢酶（CAT）活性显著低于鱼粉组（P0.05），菜子粕组血清羟自由基和丙二醛（MDA）含量显著高于鱼粉组（P0.05）。当蛋白替代16%鱼粉时，豆粕对花鲈血清生化指标、转氨酶活性及抗氧化参数的影响最小，是替代鱼粉的适宜植物蛋白源。 　　关键词：花鲈（Lateolabrax japonicus）；植物蛋白源；血清生化指标；转氨酶；抗氧化 　　中图分类号：S965.211 文献标识码：A 文章编号：0439-8114（2015）20-5087-05 　　DOI：10.14088/j.cnki.issn0439-8114.2015.20.041 　　Effects of Partial Replacement of Fish Meal by Five Plant Proteins on Serum Biochemical Indices，Aminotransferase Activities and Anti-oxidant 　　Parameters of Lateolabrax japonicas 　　FU Jing-jing，HUANG Yan-hua，CAO Jun-ming，WANG Guo-xia，MO Wen-yan，CHEN Xiao-ying，HUANG Wen-qing 　　（College of Animal Science，South China Agriculture University/Institute of Animal Science，Guangdong Academy of Agriculture Sciences/Guangdong Public Laboratory of Animal Breeding and Nutrition，Guangzhou 510640，China） 　　Abstract：A feeding experiment was conducted to investigate the effects of partial replacement of fish meal by five plant proteins on serum biochemical indices， aminotransferase activities and anti-oxidant parameters of Japanese seabass （Lateolabrax japonicus）. Four hundred and eighty juvenile fish with an initial weight of （9.69±0.15） g were randomly divided into 6 groups and fed respectively six isonitrogenous and isoenergetic diets. The basic diet contained 40% fish meal and five test diets were prepared with 16% fish meal replacement by 23.30% soybean meal， 22.40% peanut meal， 23.82% cottonseed meal， 27.77% rapeseed meal and 38.98% corn-DDGS. The feeding experiment lasted for 28 days. The results showed that there were no significant differences in serum glucose （GLU）， urea nitrogen （UN） and low density lipoprotein （LDL-C） content