依达拉奉对血管性痴呆大鼠学习记忆能力及海马ChatNR2B表达影响.docVIP

依达拉奉对血管性痴呆大鼠学习记忆能力及海马ChatNR2B表达的影响 　　[摘要] 目的:研究依达拉奉对血管性痴呆(vascular dementia,VD)大鼠学习记忆能力及海马胆碱乙酰转移酶(Chat)、N-甲基D-天门冬氨酸受体2B(NR2B)表达的影响。方法:采用“两血管阻断+硝普钠降压法”建立大鼠VD模型,采用Morris水迷宫实验对大鼠的学习记忆能力进行测试,应用免疫组化染色方法对大鼠海马Chat及NR2B的表达进行检测。结果:VD组(B组)与假手术组(A组)、依达拉奉治疗组(C组)、预防治疗组(D组)比较,学习记忆能力明显下降,海马内Chat蛋白及NR2B表达减少(P0.05)。结论:依达拉奉可以改善VD大鼠的学习记忆能力,可能由于促进海马Chat蛋白及NR2B的表达;同时,依达拉奉对大鼠认知能力的减退有预防作用。 　　[关键词] 依达拉奉;血管性痴呆;学习记忆;Chat;NR2B 　　[中图分类号] R965[文献标识码]A [文章编号]1673-7210(2010)02(c)-023-02 　　 　　Effect of Edaravone on learning, memory and expressions of hippocampal Chat and NR2B in rats with vascular dementia 　　LI Yanbo, ZHANG Shenglin 　　(Department of Geriatrics, the First Clinical Hospital of Shanxi Medical University, Taiyuan 030001, China) 　　[Abstract] Objective: To research the effect of Edaravone on learning, memory and expressions of hippocampal of Chat and NR2B in rats with vascular dementia (VD). Methods: VD rat models were established by two-vessel occlusion and Sodium Nitroprusside depressurization. Morris test was performed to detect the cognition of rats. Immunohistochemistry was used to observe the expressions of Chat and NR2B in hippocamp. Results: The learning and memory of VD group (group B) were higher compared with sham-operation group (group A), Edaravone group (group C) and preventive treatment group (group D), and the expressions of Chat and NR2B were decreased in hippocamp of group B (P0.05). Conclusion: Edaravone improves the learning and memory in VD rats, its effects may result from the increase in hippocampal Chat and NR2B expression, we can also conclude that Edaravone has a preventive effect on cognitive decline in rats. 　　[Key words] Edaravone; VD; Learning and memory; Chat; NR2B 　　 　　依达拉奉是一种新型的脑保护药,具有清除自由基、抗细胞凋亡和神经保护作用[1]。临床研究结果表明,依达拉奉在脑梗死中有显著的神经保护作用[2-3]。血管性疾呆(VD)是一组由于缺血或出血性的脑血管病及全脑缺血低氧引起的认知功能障碍综合征。本文通过观察依达拉奉对VD大鼠海马Chat、NR2B表达的影响,研究依达拉奉的预防及治疗作用,为VD的治疗提供新思路。 　　1 材料与方法 　　1.1动物分组及模型制备 　　实验动物由山西医科大学实验动物中心提供,选用雄性Wistar大鼠36只,体重200～250 g,适应性饲养2周,采用“两血管阻断+硝普钠降压法