利用TCGA数据集分析H―rasmTOR基因在膀胱癌中表达及临床意义.docVIP

利用TCGA数据集分析H―rasmTOR基因在膀胱癌中表达及临床意义 　　【摘要】 目的：研究H-ras、mTOR基因?c膀胱癌发展、预后的关系及其通路H-ras/mTOR存在的可能性。方法：从美国癌组基因组图谱（TCGA）数据库中下载膀胱癌中H-ras、mTOR基因表达谱及其临床信息资料并分析其关系，分析H-ras、mTOR基因表达的相关性。结果：（1）AJCC pTNM分期中T1～T4期（P=0.012）、T2～T4期（P=0.011）、T1～2～T3～4期（P=0.042）及病理分级（P=0.020）与H-ras的表达呈负相关（P0.05）。（2）AJCC pTNM分期中T2～T3期（P=0.042）及病理分级（P=0.014）与mTOR的表达正相关（P0.05）。（3）COX多因素分析表明mTOR是影响膀胱癌预后的独立因素。（4）H-ras、mTOR基因在膀胱癌中表达具有显著正相关（P0.05）。结论：H-ras基因可作为膀胱癌恶性程度的良性预示因素，而mTOR基因是膀胱癌恶性程度的不良预示指标，并且是影响预后的独立危险因素。两者可能通过H-ras/mTOR信号通路联系而发挥肿瘤生物学作用并有望成为膀胱癌靶向治疗的理想位点。 　　【关键词】 膀胱癌； Ras； mTOR 　　Analysis of Clinical Significance of H-ras，mTOR Gene Expression in Gastric Cancer Utilizing TCGA Datasets/WU Jian-yong，TANG Yong.//Medical Innovation of China，2017，14（11）：005-009 　　【Abstract】 Objective：To study the relationship between H-ras，mTOR gene and the prognosis of bladder cancer，and the possibility of the existence of H-ras/mTOR pathway.Method：The Gene expression profiles of H-ras and mTOR in bladder cancer and their clinical data were downloaded from the American Cancer Genome Atlas （TCGA），the relationship between H-ras，mTOR and clinical data，the correlation between the expression of H-ras and mTOR genes were analyzed.Result：（1）The expression of H-ras was negatively correlated with AJCC pTNM stage T1-T4（P=0.012），T2-T4（P=0.011），T1-2-T3-4（P=0.042） and pathological grading（P=0.020），but the invasion and metastasis prognosis weren’t associated with the level of H-ras（P0.05）.（2）The expression of mTOR was positively correlated with AJCC pTNM stage T2-T3（P=0.042），and pathological grading（P=0.014）.The patients with low expression of mTOR had longer tumor free survival time than the high expression group（P0.05）.（3）COX multivariate analysis showed that mTOR was an independent prognostic factor for bladder cancer.（4）The expressions of H-ras and mTOR gene in bladder cancer were significantly positively correlated（P0.05）.Conclusion：H-ras gene can be used as a benign prognostic factor in the malignant degree of bladder cancer，a