厚朴对大鼠心肌缺血再灌注损伤保护作用.docVIP

厚朴对大鼠心肌缺血再灌注损伤保护作用 　　[摘要] 目的 观察厚朴对大鼠心肌缺血/再灌注损伤（MIRI）的作用，并初步探讨其可能的作用机制。 方法 选择健康SD雄性大鼠26只，随机分为三组：假手术组（n=7）、模型组（n=10）、药物组（n=9），通过结扎冠状动脉左前降支30 min后再灌注120 min的方法建立大鼠心肌MIRI模型，每组随机取6只成功造模的大鼠，取其血浆及心肌组织，测定血浆肌酸激酶（CK）、髓过氧化物酶（MPO）和心肌组织中超氧化物歧化酶（SOD）的活力。 结果 模型组较假手术组CK活力下降（P0.05），MPO活力显著上升（P0.05），SOD活力下降（P0.05）；药物组较模型组CK与MPO活力均下降（P0.05），SOD活力显著上升（P0.05）。 结论 厚朴对大鼠心肌缺血/再灌注损伤具有保护作用，其作用机制可能是抗氧化损伤，抑制炎症反应，并增加细胞能量供应。 　　[关键词] 厚朴；SD大鼠；心肌缺血/再灌注损伤；保护作用 　　[中图分类号] R969 [文献标识码] A [文章编号] 1674-4721（2016）05（c）-0103-03 　　Protective effects of Mangholia officinalis on myocardial ischemia-reperfusion injury in rats 　　HU Yue 　　The Kingmed College of Laboratory Medicine，Guangzhou Medical University，Guangzhou 511436，China 　　[Abstract] Objective To investigate the effect and underlying mechanisms of Mangholia officinalis on myocardial ischemia-reperfusion injury （MIRI） in rats. Methods Twenty-six healthy male SD rats were randomly divided into three groups：sham operation group （n=7），MIRI model group （n=10），drug group （n=9）.MIRI rat models were created by reperfusion for 120 min after ligaturing anterior interventricular branch of the left coronary artery for 30 min.Then the plasma and cardiac muscle tissue of six rats of each group were collected separately，and the activities of creatine kinase （CK） and myeloperoxidase （MPO） in plasma，superoxide dismutase （SOD） in myocardium were determined. Results Compared with sham operation group，the activity of CK of model group was decreased （P0.05），the activity of MPO was increased obviously （P0.05），while the activity of SOD was decreased（P0.05）.Compared with model group，the activity of CK and MPO of drug group was decreased （P0.05），while the activity of SOD was increased obviously（P0.05）. Conclusion Mangholia officinalis has a protective effct on MIRI in rats.Its mechanism is probably associated with resisting oxidation damage，reducing inflammatory reaction and increasing the supply of energy. 　　[Key words] Mangholia officinalis；SD rat；Myocardial ischemi