吡格列酮高糖对3T3―L1细胞中p―Perilipin 1A蛋白表达影响.docVIP

吡格列酮高糖对3T3―L1细胞中p―Perilipin 1A蛋白表达影响 　　[摘要]目的 探讨吡格列酮和高糖对3T3-L1细胞中p-Perilipin 1A蛋白表达的影响。方法 将以3T3-L1前脂肪细胞诱导分化成3T3-L1脂肪细胞，进行分组，设对照组（5 mmol/L葡萄糖）、高糖组（25 mmol/L葡萄糖）、高糖加吡格列酮组（25 mmol/L葡萄糖+100 μmol/L吡格列酮），通过测定培养基中甘油含量作为评价脂肪分解的指标，同时用Western blot检测p-Perilipin 1A蛋白表达。结果 通过甘油试剂盒监测甘油释放量，高糖组较对照组明显增加，差异有统计学意义（P0.01），而高糖加吡格列酮组的甘油释放量较高糖组明显减少，差异有统计学意义（P0.01）；同时Western blot检测结果显示，与对照组比较，高糖组p-Perilipin 1A蛋白明显上调，差异有统计学意义（P0.01），高糖组与高糖加吡格列酮组比较，显著抑制了p-Perilipin 1A蛋白的上调，差异有统计学意义（P0.01）。结论 吡格列酮可能通过阻止p-Perilipin 1A上调来抑制高糖刺激下的脂肪分解，减少游离脂肪酸，从而改善胰岛素抵抗。 　　[关键词]吡格列酮；高糖；3T3-L1细胞；p-Perilipin 1A 　　[中图分类号] R589.2 [文献标识码] A [文章编号] 1674-4721（2017）04（c）-0009-04 　　[Abstract]Objective To investigate the effects of pioglitazone and high glucose on the expression of p-Perilipin 1A protein in 3T3-L1 cells.Methods 3T3-L1 preadipocytes were induced and differentiated into 3T3-L1 adipocytes and then were classified into three groups：control group （5 mmol/L glucose），high glucose group （25 mmol/L glucose）and high glucose plus Pioglitazone group （25 mmol/L glucose + 100 μmol/L Pioglitazone）.The glycerol content of culture medium was determined and taken as the evaluation index of lipolysis；at the same time，Western blot was used to detect the expression of p-Perilipin 1A protein.Results According to the release amount of glycerol monitored by using the glycerol kit，a more obvious increase was seen in high glucose group than in control group，with statistically significant difference （P0.01）；while，a more obvious decrease of the release amount of glycerol occurred in high glucose plus Pioglitazone group than that in high glucose group，with statistically significant difference （P0.01）；at the same time，the p-Perilipin 1A proteinof high glucose group detected by Western blot was significantly increased in comparison with control group，with statistically significant difference（P0.01）；and the expression of p-Perilipin 1A protein was significantly inhibited in high glucose group in contrast with high glucose plus piogl