- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
半枝莲总黄酮通过PI3KAKTmTOR通路诱导肿瘤细胞自噬体内实验研究
半枝莲总黄酮通过PI3KAKTmTOR通路诱导肿瘤细胞自噬体内实验研究
[摘要] ?研究拟从体内角度研究半枝莲总黄酮(TFSB)的抗肿瘤作用,并探讨TFSB对肿瘤细胞自噬的影响及其对PI3K/AKT/mTOR通路的调控作用,为TFSB的抗肿瘤作用提供实验依据。实验以黑色素瘤荷瘤小鼠模型为研究对象,分为空白对照组、雷帕霉素组(Rap,1.5 mg?kg-1)、TFSB高、中、低剂量组(200,100,50 mg?kg-1),每天给药1次,连续用药11 d,通过测量肿瘤的体积及抑瘤率,来观察TFSB对黑色素瘤生长的抑制效果;通过TUNEL法检测肿瘤细胞凋亡情况,来进一步验证TFSB的抗肿瘤活性;通过Western blot法检测LC3Ⅰ,LC3Ⅱ的蛋白表达,计算LC3Ⅱ对LC3Ⅰ的相对表达量,探讨TFSB对肿瘤细胞自噬的诱导作用;通过检测PI3K/AKT/mTOR通路标志蛋白的磷酸化水平,研究TFSB诱导细胞自噬的分子机制。结果表明,TFSB可有效抑制荷瘤小鼠体内黑色素瘤的生长,减小肿瘤体积、提高抑瘤率,并且可以显著增加肿瘤细胞凋亡指数,增加LC3Ⅱ/LC3Ⅰ,抑制pPI3K,pAKT及pmTOR的蛋白表达水平,与对照组比较有显著性差异(P0.05,P0.01或P0.001)。由此可见,半枝莲总黄酮具有抑制体内黑色素瘤生长的作用,其机制可能与通过抑制PI3K/AKT/mTOR通路,诱导肿瘤细胞自噬及凋亡有关。
[关键词] 半枝莲总黄酮; PI3K/AKT/mTOR通路; 自噬; 细胞凋亡
Effect of total flavonoids in Scutellaria barbata in mediating
autophagy in tumor cells via PI3K/AKT/mTOR pathway
CHEN Ming, WANG Jutao*, WU Zhenni, HU Manyan, GAO Huawu
(Department of Pharmacology, Anhui University of Traditional Chinese Medicine, Hefei 230038, China)
[Abstract] To investigate the effect of the total flavonoids in Scutellaria barbata(TFSB) against autophagy in tumor cells in vivo, and further determine whether the mechanism is correlated with the PI3K/AKT/mTOR pathway, which lead to autophagyinduced tumor cell death. Melanomabearing mice were prepared and divided into control group, rapamycin group (Rap 1.5 mg?kg-1), and high, middle and lowdose TFSB (200, 100, 50 mg?kg-1) groups. The groups were given drugs once a day for successively 11 days. The inhibitory effect of TFSB on the growth of melanoma was determined by measuring tumor volume and tumor inhibition rate. TUNEL method was used to detect the apoptosis of tumor cells to further verify the antitumor activity of TFSB. The protein expressions of LC3Ⅰ and LC3Ⅱ were detected by Western blot, and the relative expression of LC3Ⅱ was calculated based on LC3Ⅱ/LC3Ⅰ. In addition, the effect of TFSB on autophagy of tumor cells, the underlying molecular mechanism of TFSB in inducing autophagy and PI3K/AKT/mTOR pathway marker protein phospho
文档评论(0)