喉鳞癌组织中pERK和Ki67表达及其意义.docVIP

喉鳞癌组织中pERK和Ki67表达及其意义 　　[摘要] 目的：探讨p-ERK及Ki-67在喉鳞癌患者中的表达及其相关性。方法：通过免疫组化Elivison法检测65例喉鳞癌组织中p-ERK及Ki-67的表达。结果：p-ERK的阳性表达率为58.46%（38/65），其阳性表达与临床分期及临床分型有关（P0.05），与淋巴转移无关。Ki-67的阳性表达率为64.62%（42/65），其表达与临床分期及淋巴转移有关（P0.01），与临床分型无关。p-ERK与Ki-67在喉鳞癌中的表达呈正相关。结论：p-ERK与Ki-67在喉鳞癌组织中呈高表达，提示其与肿瘤的发生、发展密切相关，有望成为基因治疗的新靶点。 　　[关键词] 喉肿瘤；癌，鳞状细胞；免疫组化；p-ERK；Ki-67 　　[中图分类号] R739.65[文献标识码]A [文章编号]1673-7210（2009）05（c）-005-02 　　 　　Expression and significance of p-ERK and Ki-67 in human LSCC 　　QIN Zhigang, ZHANG Taoyuan* 　　(Department of Otolaryngology, the First Hospital of Shanxi Medical University, Taiyuan 030001, China) 　　[Abstract] Objective: To explore the expressions of p-ERK and Ki-67 and the correlation between them in patients with laryngeal squamous cell carcinoma (LSCC). Methods: The expressions of p-ERK and Ki-67 in 65 patients with LSCC were detected by Elivison immunohistochemical technique. Results: The positive rate of p-ERK expression was 58.46% (38/65). The positive expression of p-ERK was associated with the clinical stage and clinical classification (P0.05), not with the lymph node metastasis. The positive rate of Ki-67 was 64.62% (42/65), and it had a significant difference in different clinical stages and lymph node metastasis (P0.01), no significant difference in different clinical classification. The expression of p-ERK had a positive correlation with that of Ki-67 in LSCC. Conclusion: Ki-67 and p-ERK have higher expressions in LSCC, which might play an important role in the development of laryngeal carcinoma. They will be the targets of gene therapy in laryngeal carcinoma. 　　[Key words] Laryngeal neoplasm; Carcinoma; Squamous cells; Immunohistochemistry; p-ERK; Ki-67 　　 　　细胞外信号调节激酶（extracellular signal-regulated kinase，ERK）是MAPK家族中最早发现也是最重要的亚类之一，磷酸化细胞外信号调节激酶（p-ERK）是其活化形式，在应答生长刺激时具有特征性，是细胞周期相关癌基因。研究表明，p-ERK与多种肿瘤的发生、发展密切相关[1-3]。Ki-67是增殖细胞核抗原，能够反映细胞群体的增殖活性。在维持细胞有丝分裂过程中DNA的高度有序性方面发挥作用。本研究应用免疫组化Elivison方法，检测喉鳞癌（laryngeal squamous cell carcinoma，LSCC）组织中p-ERK及Ki-6