吸烟对精液质量和精子DNA完整性影响.docVIP

吸烟对精液质量和精子DNA完整性影响 　　[摘要] 目的 探讨吸烟对男性精液质量和精子DNA完整性的影响。 方法 总计197例男性不育患者（不育非吸烟者85例和不育吸烟者112例）。112例男性不育吸烟患者根据吸烟指数分为吸烟A组、B组和C组。统计分析不育非吸烟组与不育吸烟组，吸烟组A组、B组和C组之间精液质量及精子DNA完整性之间的差异。 结果 （1）不育非吸烟组与不育吸烟组前向运动精子百分率（PR）、正常形态精子百分率（MNS）、精子DNA碎片化指数（DFI）两组相比各指标均有统计学意义（P0.05）。（2）吸烟A组与B组比较，精液体积、前向运动精子百分率、精子存活率、精子浓度、正常形态精子百分率、DFI差异均无统计学意义（P0.05）；吸烟C组与A组、B组相比前向运动精子百分率、正常形态精子百分率显著下降，DFI指数显著升高，差异有统计学意义（P0.05）。 结论长期大量吸烟可导致男性精液质量下降和精子DNA的损伤，从而影响男性生育。 　　[关键词] 吸烟；男性不育症；精液质量；精子DNA完整性 　　[中图分类号] R446.1 [文献标识码] A [文章编号] 1673-9701（2015）26-0012-03 　　Effects of cigarette smoking on semen quality and human sperm DNA integrity 　　HONG Fei CHEN Jianwei LOU Jiangtao ZHANG Xiaoxia 　　Department of Clinical Laboratory， Ningbo Hospital of Traditional Chinese Medicine Ningbo 315012， China 　　[Abstract] Objective To explore the effects of cigarette smoking on semen quality and the sperm DNA integrity. Methods A total number of 197 cases of male infertility patients（85 cases infertile of non-smokers and 112 infertile of smokers） were collected. According to smoking index， 112 cases of male infertility smokers were divided into group A、 group B and group C. The differences in semen quality and sperm DNA integrity were statistically analyzed between the infertile non-smoker group and infertile smoker group， also between smoking group A， group B and C. Results There were statistically significant differences between the infertility non-smoker group and infertile smoker group， with the rate of progressively motile sperm （PR）， the rate of morphologically normal sperm （MNS） and the DNA fragmentation index（DFI）， respectively. The sperm concentration， sperm viability rate， semen volume had no significant difference（P0.05）. （2）There was no statistically significant difference between the smoking group A and group B in semen volume， PR， sperm viability rate， sperm concentration， MNS and DFI（P0.05）. Compared with smoking group A and B， the PR and MNS of group C were decreased obviously， meanwhile the DFI was increased